飞行时间质谱(TOFMS)操作规程.pdfVIP

飞行时间质谱操作规程 一，靶板清洗程序 1. 用丙酮冲洗靶板直到去除所有可见的样品残余 2. 用 1%甲酸溶液超声 10 分钟 3. 用去离子水超声 10 分钟 4. 用分析纯丙酮超声 10 分钟 5. 用分析纯甲醇超声 10 分钟 6. 放入盒中，自然干燥 二，样品制备 基质选择及配置标准溶液 本实验室现有基质如下： 3-Indoleacetic acid （IAA ），3-Hydroxypicolinic acid （HPA ），Trihydroxyanthracene （DI ）， 2,5-Dihydroxybenzoic acid （DHB ），2,4,6-Trihydroxyacetophenone monohydrate （THAP ）， 2,6-Dihydroxyacetophenone （DHAP ），2-(4-Hydroxyphenylazo)benzoic acid (HABA)， 根据测试人样品特点，参考相应文献报道，选择合适基质（以下表格仅供参考） Gentisic acid, Aldrich 14,935-7 Dissolve 10 mg in 1 mL of Most samples such as 2,5-Dihydroxybenzoic acid (DHB) mixed solution of acetonitrile protein, DNA, sugar, (C H O :154.1) and 0.1% TFA (2:3). lipid and synthetic high 7 6 4 polymer For 1 mg of high polymer, Polar synthetic high dissolve 10 mg and 1 mg of polymer (All become NaCl, LiCl or KCl in Na, Li or K added ions.) demineralized water. Dissolve 108 mg and 16 mg of Single stranded DNA, ammonium citrate dibasic in a RNA of 50 mer or more 50% acetonitrile solution. Dithranol, Sigma D-2773 For 1 mg of high polymer, Non-polar synthetic 1,8-Dihydroxy-9[10H]-anthraceno dissolve 10 mg in chloroform or high polymer ne THF. (C H O :226.2) For 1 mg of high polymer, Non-polar synthetic 14 10 3 dissolve 10 mg and 1 mg of high polymer (All silver trifluoroacetate in become Ag added ions.) chloroform or