依达拉奉预给药对油酸致急性肺损伤大鼠作用及机制.docVIP

依达拉奉预给药对油酸致急性肺损伤大鼠的作用及机制 　　[摘要] 目的 探讨依达拉奉预先给药后，对油酸致急性肺损伤（acute lung injure，ALI）模型大鼠的作用及机制。 方法 雄性SD大鼠30只，随机分为3组（n = 10）：对照组（A组）腹腔注射10 mL/kg的0.9%氯化钠注射液后，鼠尾静脉注射0.9%氯化钠注射液0.1 mL/kg；ALI组（B组）腹腔注射10 mL/kg的0.9%氯化钠注射液后，静脉注射油酸0.1 mL/kg制备ALI模型；依达拉奉组（C组）腹腔注射10 mL/kg的依达拉奉后同B组方法建立ALI模型。6 h后处死大鼠，抽取腹主动脉血液行血气分析测定血氧分压（PO2），用ELISA法测定血液中肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）、一氧化氮（NO）的含量及肺组织中超氧化物歧化酶（SOD）的含量，分离大鼠右肺中叶，测定肺组织湿/干重比（W/D）。 结果 与A组相比，B、C组大鼠腹主动脉血PO2、肺组织SOD含量均低于A组，差异有统计学意义（P 0.05）。C组PO2、SOD低于A组，但是其降低的幅度小于B组，与B组相比，C组PO2、SOD高，且差异有统计学意义（P 0.05）。A组W/D、TNF-α、NO、MDA的含量均低于B、C组；B组W/D、TNF-α、NO、MDA的含量均增加明显，与A组比较，差异有统计学意义（P 0.05）；C组W/D、TNF-α、NO、MDA的含量增加幅度小，与B组比较，差异有统计学意义（P 0.05）。 结论 预先注射依达拉奉不能减少肺损伤的发生率，但可减轻油酸诱导的大鼠急性肺损伤程度，其机制可能与清除氧自由基和抑制炎性因子释放有关。 　　[关键词] 急性肺损伤；油酸；自由基清除剂；依达拉奉 　　[中图分类号] R563 [文献标识码] A [文章编号] 1674-4721（2012）03（a）-0019-02 　　 　　Effects of edaravone pretreatment on acute lung injury in rat model of injected oleic acid 　　SUN Hui1 LIU Yongming2 　　1.Department of Laboratory, the Third Peoples Hospital of Taiyuan City in Shanxi Province, Taiyuan 030012, China; 2.Department of Laboratory, the Second Peoples Hospital of Shanxi Province, Taiyuan 030012, China 　　[Abstract] Objective To investigate the effect of edaravone pretreatment on acute lung injury in rat model of injected oleic acid. Methods Thirty SD rats were randomly divided into 3 groups (n = 10): group A (sham operated group), group B (ALI group), group C (edaravone group). Group A received intraperitoneal injection of normal saline 10 mL/kg, than prepared model by intravenous injection normal saline 0.1 mL/kg. Group B received intraperitoneal injection of normal saline 10 mL/kg, than prepared model by intravenous injection OA 0.1 mL/kg. Group C received intraperitoneal injection of edaravone 10 mL/kg, than prepared model by intravenous injection OA 0.1 mL/kg. PO2 of blood in the aorta abdominalis were measured. The expression of TNF-α, NO, MDA, SOD was examined by ELISA. Wet/dry weight of lung tissue was calculated. Results The