克氏原螯虾消化系统组织学及超微结构研究.docVIP

克氏原螯虾消化系统组织学及超微结构研究 　　摘 要:应用石蜡切片和电镜技术对克氏原螯虾消化系统的胃、中肠、后肠及肝胰腺等进行了组织学和超微结构研究。结果表明,克氏原螯虾的消化系统由消化道和消化腺组成。胃的柱状上皮层上覆盖几丁质层,贲门胃内具有特化结构-胃磨,幽门胃表面密集的刚毛深入胃腔。肠的柱状上皮层向肠腔内突起构成肠嵴,表面有棘刺,后肠的上皮层上还具有几丁质层。肝胰腺由指状肝小管紧密排列构成的多分支肝盲管组成,上皮细胞由F细胞、B细胞、R细胞和E细胞4种类型细胞构成,R细胞的细胞质中含有脂滴和吞噬泡以及少量的内质网和线粒体;F细胞的细胞质中粗面内质网发达;B细胞内有许多大小不一的液泡和消化泡;E细胞体积小,核质比大,细胞质中内质网和线粒体等细胞器数量少。克氏原螯虾消化系统的组织结构特征与其功能相一致。 　　关键词:克氏原螯虾; 消化系统; 组织学; 超微结构 　　Studies on hlstology and ultrastructure of the digestive system of freshwater crayfish, procambarus clarkii 　　Song Xin[1,2] Wei Ke-qiang[2] Xu Zi-rong[3] 　　( 1. Zhuozhou Branch School of Baoding Teachers College, Zhuozhou 072750, China; 　　2. School of Life Science, Shanxi University, Taiynan 030006, China; 　　3. College of Animal Sciences, Zhejiang University, Hangzhou, 310029, Zhejiang, China ) 　　Abstract:The structure of the digestive system of freshwater crayfish, Procambarus clarkii, was studied with light and electronic microscopy. The digestive system consisted of digestive tract and hepatopancreas. The columnar epithelial surface of the stomach was covered by cuticle. The cuticle in the cardiac stomach evolved into gastric mill and seta were observed on the surface of phloric stomach. There was intestinal ridge from the columnar epithelial surface of the gut and spines were observed on the surface of the gut. The epithelial surface of the hind-gut was covered by cuticle also. The hepatopancreas was tubular type gland.According to morpha and function of epithlium cell, they could be divided into four type cells (E, R, F, B). R-cells were characterized by lipid droplet and phagosoma. There were abundant rough endoplasmic reticulum in the F-cells and a large number of vacuoles in B-cells. E-cells were small and showed a high radio of nuclear to cytoplasm. The cytoplasm contained relatively sparse rough endoplasmic reticulum and small mitochondria. These structure features of digestive system of Procambarus clarkii were identical to their function. 　　Keywords: Procambarus c