耐锑菌株在修复洞庭湖复合污染土壤中的应用研.docVIP

耐锑菌株在修复洞庭湖复合污染土壤中的应用研究 摘要 近年来，负有“鱼米之乡”的洞庭湖由于湖区居民排放的生活垃圾、工业生产排弃的固体废弃物以及水产养殖业中不规范投饵所造成的污染物大肆囤积于湖区土壤，造成当地土壤呈复合污染趋势，给当地居民的生产生活带来极大地不便。其中危害最强、治理难度最大的就属复合污染中的中金属污染，对于重金属污染的治理方法主要包括化学、物理及生物方法，而具有修复能力强、费用低、无二次污染的生物治理法也逐渐受到研究者的关注，很多植物修复技术也日渐成熟，但植物修复技术自身也存在很多缺陷，如植株个体矮小、生长缓慢、根系扩张能力有限、对金属有特异性、转移率低等。研究表明植物修复效率的提高很大程度依赖于重金属种类、突然根际微生物和植物本身之间的相互作用。Glick认为植物根部跟土壤中大量微生物之间的相互作用是觉得修复程度的主要决定因素。 本研究基于以上理论基础，以治理研究较少的锑污染为目的，在锑污染严重的矿区分离耐锑菌株，并将分离纯化后的菌株接种到污染土壤中，评价耐锑菌株对洞庭湖复合污染土壤的修复及耐锑菌株与蒌蒿共同作用对土壤修复的的效果。 对分离纯化得到的菌株进行形态学的观察，结果表明该菌株为青霉菌；研究结果表明该菌株对锑的吸附力随锑初始质量浓度的增大而减少，在锑质量浓度大于600g／L时其生长受到抑制，对锑的最高吸附率为64%；不同浓度的锑均不会影响土壤理化性质；另外该菌株可增强蒌蒿对土壤锑的吸附能力。 关键词: 耐锑菌株 修复 洞庭湖 土壤污染 Research on the application of antimony- resistant bacterial strain to repairing soil pollution of Dongting Lake in China Abstract In recent years, the Dongting Lake, which is famous for the land of fish and rice, is polluted by round residents emissioning garbage, the abandoned solid waste in industrial production and aquaculture with no regulated feeding , hoarding in the district soil, resulting in great inconvenience to the life of the local residents. The strongest harm to the soil is the metal pollution in the complex pollutants. Now the way to manage the heavy metal pollution methods includes chemical, physical and biological methods, which gradually was taken attention by researchs for the ability to repair, low cost, no secondary pollution bioremediation. A lot of phytoremediation technology have become more mature, but the method of phytoremediations have many defects, such as individual plants short stature, slow growth, root expansion capacity is limited, metal specificity, low transfer rate . Studies have shown that the phytoremediation efficiency is strongly dependent on the type of heavy metal, suddenly the root of the interaction between the occasion of microorganisms and plants. Glick plant roots with a large number of micro-organisms in the soil interaction between think the main determinants of the level of repair. Thi