冰片联合灯盏花素对缺氧复氧损伤血脑屏障通透性影响.docVIP

冰片联合灯盏花素对缺氧复氧损伤血脑屏障通透性影响 　　摘要：目的 观察冰片联合灯盏花素对缺氧/复氧下血脑屏障（BBB）通透性的影响，探讨其保护BBB的作用机制。方法 培养原代大鼠脑微血管内皮细胞（BMEC），在缺氧培养箱中缺氧2 h，再复氧24 h，建立单层BMEC体外BBB模型。实验分为正常对照组、模型组、冰片组、灯盏花素组、冰片+灯盏花素组，各给药组给予相应药物干预，检测各组跨内皮细胞电阻（TEER）值和辣根过氧化物酶（HRP）的通透性，ELISA、Western blot检测BMEC中紧密连接蛋白ZO-1及Claudin-5蛋白表达。结果 缺氧/复氧损伤导致TEER值降低，HRP通透率增加，ZO-1及Claudin-5蛋白表达降低；冰片联合灯盏花素可明显改善上述损伤。结论 冰片联合灯盏花素对缺氧/复氧下BBB的损伤具有保护作用，其机制可能与上调ZO-1、Claudin-5蛋白表达有关。 　　关键词：冰片；灯盏花素；血脑屏障；大鼠 　　DOI：10.3969/j.issn.1005-5304.2016.02.021 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）02-0076-03 　　Effects of Borneolum Syntheticum Combined with Breviscapine on Blood-brain Barrier Permeability Induced by Hypoxia/Reoxygenation Injury XU Lu1， ZHANG Tai-jun2 （1. Key Lab of Biochemical and Molecular Pharmacology， Chongqing Medical University， Chongqing 400016， China； 2. Chongqing City Hospital of Traditional Chinese Medicine， Chongqing 400021， China） 　　Abstract： Objective To observe effects of Borneolum Syntheticum combined with breviscapine on blood-brain barrier （BBB） permeability induced by hypoxia/reoxygenation injury. Methods The BBB model in vitro was established by primarily culturing brain microvascular endothelial cells （BMEC）， hypoxia 2 h and reoxygenation 24 h. The experiment was divided into normal control group， model group， Borneolum Syntheticum group， breviscapine group， and Borneolum Syntheticum-breviscapine group. All treatment groups were given relevant medicine. TEER and HRP were detected； ELISA and Western blot was used to detect the expressions of ZO-1 and Claudin-5 in BMEC. Results Hypoxia/reoxygenation induced injury decreased TEER， increased permeability rate of HRP， and decreased the expressions of ZO-1 and Claudin-5； while Borneolum Syntheticum combined with breviscapine could improve damage caused by hypoxia/reoxygenation. Conclusion Borneolum Syntheticum combined with breviscapine shows obvious protective effect on BBB permeability induced by hypoxia/reoxygenation， and the mechanism is involved in the regulation of ZO