中心体相关激酶Aurora A和mt P53在大.docVIP

　　中心体相关激酶Aurora A和mt P53在大 【摘要】 　　目的： 研究中心体相关激酶AuroraA和突变型P53(mtP53)在大肠癌中的表达规律，探讨其相互关系及在肿瘤发生发展中的作用. 方法： 应用组织芯片和免疫组化技术（SP法）检测AuroraA和mtP53在51例大肠癌组织、癌旁组织和正常大肠组织中的表达，并结合临床病理学参数进行综合分析. 结果： 在正常大肠组织、癌旁组织和大肠癌组织中AuroraA的阳性表达率分别为0，36％和69％；mtP53的阳性表达率分别为0，21％和57％. 与正常大肠组织和癌旁组织相比，大肠癌组织中AuroraA和mtP53的表达明显升高（Plt;0.05）；两者的表达与肿瘤的浸润程度关系密切（Plt;0.05），mtP53还与Dukes分期和淋巴结转移有关（Plt;0.01）. AuroraA在大肠癌组织中的表达与mtP53表达呈显著正相关（r= 0.776, Plt;0.01）. 结论： AuroraA蛋白的过表达与P53的突变在大肠癌的发生、发展中发挥着重要作用. AuroraA和mtP53蛋白的表达对大肠癌的早期诊断及预后判断具有重要价值. 【关键词】 AuroraA；基因，P53；基因组不稳；组织芯片；结直肠肿瘤 【Abstract】AIM: To investigate the expression levels of centrosomeassociated kinase AuroraA and mutant type P53(mtP53) in colorectal cancer and to explore their mutual relationship and their roles in tumorigenesis and tumor progression. METHODS: Pathological specimens from colorectal cancer, pericancerous tissues and normal colorectal tissues ined for the expression levels of AuroraA and mtP53 proteins by tissue microarray technique and immunohistochemistry (SP method), and the clinicopathological parameters al colorectal tissues, pericancerous tissues and colorectal cancer tP53 expression in normal colorectal tissues, pericancerous tissues and colorectal cancer al colorectal tissues and pericancerous tissues, the expression levels of AuroraA and mtP53 or tissues (Plt;0.05). AuroraA overexpression tP53 overexpression ph node metastasis and Dukes classification (Plt;0.05). There tP53(r=0.776, Plt;0.01). CONCLUSION: Overexpression of AuroraA and mutation of P53 play a critical role in tumorigenesis and tumor progression of colorectal cancer. So codetection of AuroraA and mtP53 may be useful for early diagnosis and prognosis prediction of colorectal cancer. 【Keyic instability; tissue microarray; colorectal neoplasms 　　0引言 　　有丝分裂过程的偏差会导致基因组不稳定从而引发肿瘤. 研究发现，AuroraA是维持基因组稳定性所必需的、关键的有丝分裂调节因子，这个中心体相关激酶在肿瘤细胞中过度表达［1］. 突变型P53(mtP53)在维持基因组稳定方面同样发挥着重要作用，它参与DNA的复制和修复. 我们利用组织芯片和免疫组化技术检测AuroraA和突变型mtP53蛋白在