壳聚糖对胃溃疡大鼠血清中MDASOD及GSHPX影响.docVIP

壳聚糖对胃溃疡大鼠血清中MDASOD及GSHPX影响 　　摘 要 目的：观察壳聚糖对大鼠消化性溃疡愈合的影响，探讨其抗溃疡的可能机制。方法：采用冰乙酸胃窦部浆膜下注射法制备大鼠消化性溃疡模型。70只实验大鼠被随机分成7组，即假手术对照组、模型对照组、泮托拉唑治疗组、硫糖铝混悬液治疗组及3个不同剂量壳聚糖治疗组（ 0.25 g/kg,0.5 g/kg,1.0 g/kg）。按Guth标准记分判定溃疡指数并计算溃疡抑制率；HE染色观察组织结构。采用羟胺法测定超氧化物岐化酶(SOD)，DTNB法测定谷胱甘肽-过氧化物酶(GSH-Px)，TAB法测定丙二醛(MDA)。结果：壳聚糖治疗组与模型对照组比较，溃疡指数明显降低，溃疡抑制率升高（P 0.01)；1.0 g/kg壳聚糖治疗组大鼠血清SOD、MDA、GSH-Px值与模型对照组均有显著性差异（P?0.05）； 0.25 g/kg壳聚糖治疗组及0.5 g/kg壳聚糖治疗组大鼠血清MDA值与模型对照组比较均有显著性差异（P ?0.05），但SOD、GSH-Px值与模型对照组比较均无显著性差异。结论：壳聚糖可促进冰乙酸溃疡大鼠的溃疡愈合，并呈一定的量效关系。其促进溃疡愈合作用可能与抗氧化机制有关。 　　关键词 胃溃疡 壳聚糖 抗氧化作用 　　中图分类号：R97 文献标识码：B 文章编号：1006-1533(2008)05-0219-04? 　　 　　Effect of chitosan on the serum levels of MDA, SOD, and GSH-Px in rats with gastric ulcer? 　　Qu Chunying, Li Dingguo, Wang Yuqin, Chen Meimei? 　　(Xinhua Hospital of Medical Instite of Shanghai Jiaotong University, Shanghai，200092)? 　　ABSTRACT Aim: 　　To observe the effect of chitosan on the healing of gastric ulcer and explore the possible mechanisms for its action. Methods: Glacial acetic acid was subserosally injected to the gastric antrum of rats to establish the animal model of gastric ulcer. Seventy rats were randomly divided into 7 groups: sham-operated group, model group, pantoprazole-treated group, sucralfate-treated group, and three chitosan-treated groups at low, moderate and high doses (0.25, 0.5, and 1.0 g/kg). The ulcer index was evaluated by Guth method and the inhibition ratio of ulcer formation was calculated. The morphological changes were observed by HE staining. The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px),and malondialdehyde (MDA) were determined by hydroxylamine method, DTNB method and TAB method respectively. Results: The ulcer index was significantly decreased and the inhibition ratio of ulcer formation was significantly increased in chitosan-treated groups compared with model group (P0.01). The serum levels of MDA, SOD and GSH-Px in chitosan 1.0 g/kg group had significant difference compared with those of model gr