孕酮对妊娠期子宫平滑肌收缩功能及细胞内钙离子作用临床研究.docVIP

孕酮对妊娠期子宫平滑肌收缩功能及细胞内钙离子作用临床研究 　　【摘要】目的：探究与分析孕酮对妊娠期子宫平滑肌收缩功能及细胞内钙离子作用的影响。方法：选取我院2014年2月至2014年10月入院的足月妊娠妇女60例，其中足月临产30例，足月未临产30例。两组中采取随机数字表法各分为两组，即：A组：无催产素诱导的足月未临产组；B组：无催产素诱导的足月临产组；C组：催产素诱导足月未临产组；D组：催产素诱导的足月临产组。 将孕酮以浓度递进的方式加入已经建立规律子宫肌宫缩的实验槽中，观察子宫肌条收缩情况。同时观察免疫显光显微镜测定细胞内钙离子浓度在不同孕酮浓度和孕酮作用时间下的变化。结果：A组和B组中不同浓度的孕酮对子宫收缩波下面积的抑制率与未加孕酮时相比无统计学差异（P005），C组和D组中不同浓度的孕酮对子宫收缩波下面积的抑制率与未加孕酮时相比有统计学差异（P005）。孕酮浓度不同的实验组与对照组相比较，平滑肌细胞内钙离子浓度升高均降低，差异具有统计学意义（P005），孕酮作用时间不同的实验组与对照组相比可发现细胞内钙离子染色剂浓度升高幅度明显降低，差异具有统计学意义（P005）。结论：孕酮对妊娠子宫平滑肌收缩功能机有抑制作用及降低平滑肌细胞内钙离子浓度的作用。 　　【关键词】孕酮；子宫平滑肌 ；钙离子浓度 　　Effect of progesterone on uterine smooth muscle contraction and intracellular calcium ion during pregnancyXU Yunfang1， LIU Xiaohua1， DENG Wenping2. 1.Department of Obstetrics and Gynecology， Fuling Center Hospital of Chongqing， Chongqing 408000， China； 2.ClinicalLaboratory， Fuling Center Hospital of Chongqing， Chongqing 408000， China 　　【Abstract】Objectives： To explore and analyze the impact of progesterone on uterine smooth muscle contraction and intracellular calcium ion during pregnancy. Methods： 60 full-term pregnant female in our hospital from February 2014 to October 2014 were selected including 30 full-term labor cases and 30 cases of full-term but not in labor. Both groups， using a random number table， were divided into two groups again. Group A： no oxytocin induced full term but not in labor； group B： no oxytocin induced full-term labor； group C： oxytocin-induced full-term but not in labor； group D： oxytocin induced full-term labor. The progesterone was given in a concentration progressive way to the experimental tank with regular uterine muscle contractions， observing the uterine muscle contraction situation. Meanwhile， the light microscope was used to measure the immune significant intracellular calcium concentration changes at different concentrations of progesterone and progesterone action time. Results： Compared with no progesterone， the area under the contraction wave of inhibition of uterine con