TLR4介导的信号通路基因多态性与2型糖尿病遗传易感性的关联研究-流行病与卫生统计学专业论文.docxVIP

暨南大学 暨南大学硕士学位论文 TLR4 介导的信号通路基因多态性与 2 型糖尿病遗传易感性的关联研究 PAGE PAGE VI Abstract Objective Type 2 diabetes was closely associated with immune and inflammation. It is one of a significant health problem worldwide. However, the pathogenesis of T2DM has not yet been fully elucidated. Toll like receptor 4 was considered an important receptor that plays a key role in development of insulin resistance and complication. Animal experiments showed that activation of TLR4 and downstream cytokine production lead to the development of diabetes. But it is still unknown whether this signaling pathways genetic variation is associated with T2DM. The aim of this study was to investigate whether the polymorphisms in TLR4, MyD88, IRAK4 and TRAF6 gene are associated with T2DM. Methods This was a matching case-control study. A total of 1106 subjects were included in the study. The population included 553 subjects with T2DM and 553 control subjects. Genomic DNA was extracted from peripheral whole blood samples. All clinical information was collected in cases and controls. All Single nucleotide polymorphisms (SNPs) in the TLR4, MyD88, IRAK4 and TRAF6 gene on human chromosomes were from the human genome database. Preliminary experiments to determine the SNPs in TLR4, MyD88, IRAK4 and TRAF6 genes. All SNP genotyping using the Sequenom MassARRAY iPLEX platform. Associations between the analyzed the polymorphisms which in TLR4, MyD88, IRAK4, TRAF6 gene and T2DM were evaluated. All of the statistical analyses were performed with Statistical Product and Service Solutions software 13.0. Results Selected a total of 15 SNPs in the four genes, including TLR4 gene (rs1927914, rs1927911, rs1927907, rs2149356, r rs7873784 and rs1927906), MyD88 gene (rs7744, rs6853), IRAK4 gene (rs4251569, rs1461567, r rs4251532) and TRAF6 gene (r rs5030445). There were significant differences between cases and controls in body mass index, low density lipoprotein, high density lipoprotein and