分子生物学实验原理和技术论文.docVIP

CHITOSAN ON ANDRAEANUM TISSUE INHIBITION OF BACTERIA CONTAMINATION Abstract: The fungi was isolated and purified from polluted anthurium subculture medium, identified to be DeuteromycotinaHyphpmycetes Hyphomycetales Dematiaceae and trichoderma. The inhibition effect of the three kinds of chitosan on the fungi wasconducted.The results showed that the chitosan had inhibition effect on the fungi. The higher concentrations of chitosan had a betteinhibition effect than the lower ones.The inhibition effect of 50000 and molecular weight chitosan is better than that of the3000 molecular weight. It is preliminarily proved that chitosan had good inhibition effect on the fungi from polluted tissue culture , andwill have good application value in the fields of pollution prevention in flowers tissue culture. Key words: tissue culture； contamination； Chitosan； inhibition effect Plant tissue culture, not only in plant breeding, rapid propagation of seedlings and the production of useful secondary metabolites, such as the use of a wide range of plant genetic engineering is and Plant Molecular Biology, one of the important foundation for the study [1] . Pollution, browning, glass and tissue culture are the main problems, including pollution of the most common [2].Therefore take effective prevention and control measures to reduce the chance of contamination occurred, tissue culture is an important guarantee for success. Chitosan (chitosan) is the chitin in strong alkaline conditions from B. After the formation of acyl an important derivative is determined by a number of N-acetyl glucosamine through β-(1-4) glycosidic bond linking the nature of 18 it more lively. Has natural antibacterial properties of chitosan, and a broad spectrum antimicrobial , in recent years, chitosan in various fields such as agriculture, medicine [3], manufacture [4], food [5] , etc. The use of great importance. Tissue culture of the chitosan in the application of inhibition has not been reported. This exp