水煎提取及浓缩工艺对感冒退热颗粒有效成分影响.docVIP

水煎提取及浓缩工艺对感冒退热颗粒有效成分影响 　　摘要：目的 考察感冒退热颗粒处方药材在水煎提取、减压浓缩、常压浓缩过程中有效成分表告依春、连翘酯苷A、连翘苷转移率的变化。方法 感冒退热颗粒处方药材水煎提取后，分别进行减压浓缩和常压浓缩，采用高效液相色谱法（HPLC）测定不同状态下有效成分表告依春、连翘酯苷A、连翘苷的含量，计算转移率。结果 水煎提取后，表告依春、连翘酯苷A、连翘苷的转移率分别为72.97%、29.27%、72.84%，减压浓缩过程中3种指标性成分转移率RSD2%，浓缩至相同状态时，常压浓缩表告依春、连翘酯苷A、连翘苷的转移率分别比减压浓缩降低了13.51%、7.38%、4.24%。结论 本研究建立了同时检测表告依春、连翘酯苷A、连翘苷的HPLC方法，感冒退热颗粒的制剂工艺应据此选择合理的提取和浓缩工艺。 　　关键词：感冒退热颗粒；水煎提取；浓缩；有效成分 　　DOI：10.3969/j.issn.1005-5304.2016.01.022 　　中图分类号：R283.5 文献标识码：A 文章编号：1005-5304（2016）01-0093-03 　　Effects of Water-extracting and Concentration Process on Active Ingredients of Ganmao Tuire Granules YANG Bei-bei1， WANG Bao-hua1， LI Ping1， JI Wen-qin1， MENG Qing-qing1， SHI Jin-bo1， LI Li-juan2 （1. School of Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 100102， China； 2. Shexian Chinese Medicine Hospital， Shexian 056400， China） 　　Abstract： Objective To observe the changes in transfer rates of epigoitrin， forsythoside A and forsythin in Ganmao Tuire Granules prescription medicinal materials by water decoction extraction， vacuum concentration and atmospheric concentration. Methods Prescription medicinal materials from Ganmao Tuire Granules were decocted in water and the decoction was condensed by vacuum and atmospheric concentration process. The contents of epigoitrin， forsythoside A and forsythin at different states were measured by HPLC for transfer rates. Results The transfer rates of epigoitrin， forsythoside A and forsythin were 72.97%， 29.27%， 72.84%， respectively after decocted in water. The RSD of epigoitrin， forsythoside A and forsythin were less than 2% in the process of vacuum concentration. The transfer rates of epigoitrin， forsythoside A and forsythin by atmospheric concentration decreased by 13.51%， 7.38% and 4.24% respectively compared with vacuum concentration when concentrated to the same state. Conclusion The method for simultaneous determination of epigoitrin， forsythoside A and forsythin by HPLC has been established. A more suitable