水通道蛋白5钠泵α1亚单位在慢性阻塞性肺疾病患者气道表达改变及临床意义.docVIP

水通道蛋白5钠泵α1亚单位在慢性阻塞性肺疾病患者气道表达改变及临床意义 　　[摘要] 目的：通过测定慢性阻塞性肺疾病（COPD）患者肺组织中水通道蛋白5（AQP）、钠泵α1亚单位蛋白的表达及与肺功能变化的关系，明确AQP5、钠泵α1亚单位在COPD发生发展中的作用。方法：选取肺叶切除术患者肺组织标本52例，其中，对照组25例，COPD组27例；免疫组化法检测AQP5、钠泵α1亚单位蛋白表达。结果：与对照组相比，AQP5蛋白在COPD组表达降低（P＜0.05），在COPD中度组较轻度组表达亦降低，两组比较，差异有统计学意义（P＜0.05）。AQP5表达改变与FEV1/FVC、FEV1预计值呈正相关关系（Rs=0.717，P＜0.05；Rs=0.500，P＜0.01）。钠泵α1亚单位在COPD组患者的表达与对照组相比差异无统计学意义（P0.05）。结论：AQP5参与了COPD患者气道黏液分泌的病理过程并与COPD气流受限有关，钠泵α1亚单位在COPD的发病机制中可能起辅助作用。 　　[关键词] 慢性阻塞性肺疾病；水通道蛋白5；钠泵；黏液纤毛功能障碍 　　[中图分类号] R563 [文献标识码] A [文章编号] 1673-7210（2011）11（c）-031-03 　　 　　Changes and clinical significance of the expression of Aquaporin 5 and sodium pump α1 subunit in the airway of patients with chronic obstructive pulmonary disease 　　SHI Zhihong1, ZHANG Huifeng2 　　1.Department of Respiration Medicine, the First Affiliated Hospital of Medical College in Xi′an Jiaotong University, Shaanxi Province, Xi′an 710061, China; 2.Department of Intensive Medicine (ICU), He′nan Provincal People′s Hospital, Zhengzhou 450003, China 　　[Abstract] Objective: To study the role of AQP5 and sodium pump α1 subunit in development of COPD by measuring AQP5 and sodium pump α1 subunit protein expression and their relationships with pulmonary function changes in COPD patients. Methods: The sample from 52 cases of pulmonary lobectomy patients, including 25 cases of control group and 27 cases of COPD group, each sample was stained by immunohistochemical staining to detect AQP5 and sodium pump α1 subunit protein expression. Results: Compared with the control group, AQP5 protein expression decreased in the COPD group (P0.05). Conclusion: AQP5 participates in pathological process of airway mucus secretion in COPD patients and has the relationgship with airway obstruction of COPD; sodium pump α1 subunit may play a minor role in the pathogenesis of COPD. 　　[Key words] Chronic obstructive pulmonary disease; Aquaporin 5; Sodium pump; Mucociliary dysfunction 　　 　　慢性阻塞性肺疾病（chronic obstructive pulmonary diseases，COPD）是常见的慢性呼吸