新生大鼠脑白质损害时神经胶质细胞凋亡与FascAS Pase3蛋白的表达变化.doc

　 论文范文 　 　 题目：新生大鼠脑白质损害时神经胶质细胞凋亡与Fas、caspase3蛋白的表达变化 　 编辑： HYPERLINK /wangguiqing1987 司马小 　 　 作者：覃琳，熊英，卓平辉，阳倩 【摘要】 目的 探讨Fas、caspase3在新生大鼠脑白质损害时神经胶质细胞凋亡中的作用。方法 实验组和对照组各45只大鼠分别于缺氧缺血后30min、1h、4h、12h、1d、3d、7d、14d及21d处死，免疫组化(SP)法检测Fas、caspase3蛋白表达，TUNEL法测定细胞凋亡。结果 新生大鼠脑白质损害时，细胞凋亡指数在缺氧缺血后4h开始显著增加，3d达到高峰(P0.05)。对照组未检测到Fas，实验组Fas蛋白在缺氧缺血后30min开始出现，1h增加，12h 达高峰，并持续至3d(P0.05)。实验组caspase3蛋白表达上调，于缺氧缺血后1d达到高峰，与对照组相比在1h、4h、12h、1d、3d、7d有统计学意义(P0.05)。结论 新生大鼠脑白质损害时，Fas、caspase3表达及凋亡指数显著增加，并有明显时序性，提示Fas途径激活caspase3后引起的细胞凋亡可能是新生大鼠脑白质损害的发病机制之一。 【关键词】 脑白质损害；脑室周围白质软化；Fas；caspase3；凋亡 　　Expression of Fas, caspase3 protein and change of apoptosis　in neonatal rats with white matter damage 　　ABSTRACT: Objective To explore the effect of Fas and caspase3 on apoptosis in the white matter damage (WMD) neonatal rats. Methods The pups (45 in each group) were perfused at 30min, 1h, 4h, 12h, 1d, 3d, 7d, 14d, 21d of recovery from hypoxiaischemia (HI). Immunohistochemical technique was applied to investigate the changes in the expression of Fas and caspase3 in periventricular white matter tissues. Apoptotic cells were detected in these tissues by TUNEL. Results Apoptosis index (AI) in the experimental group increased significantly at 4h and reached the peak at 3d after HI. No expression of Fas was found in the control group. The expression of Fas in the experimental group appeared at 30min after HI, increased at 1h, reached the peak at 12h and lasted till 3d. Caspase3 in the experiment group was upregulated, peaked at 1d, demonstrating significant differences at 1h, 4h, 12h, 1d, 3d and 7d compared with the control group (P0.05). Conclusion In the WMD neonatal rats, the expression of Fas and caspase3 and AI increased significantly in turn, suggesting that the Fas signaling pathway activated caspase3 results in apoptosis may participate in the pathogenesis of WMD. 　　KEY WORDS: white matter damage (WMD); periventricular leukomalacia; Fas; caspase3; apoptosis 　　随着早产儿出生率及存活率的增加,早产儿脑损害的发病率亦逐年