慢病毒颗粒制备及应用0524.pptVIP

Outline 体细胞定向重编程的研究思路 慢病毒的构建原理 慢病毒颗粒制备方法及应用 Application of lentivirus in somatic reprogramming Vierbuchen T, et al.(2010) Direct conversion of fibroblasts to functional neurons by defined factors. Nature 463:1035-1041 Lijian H, et al. (2011) Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors. Nature 475:386-389 Edward E. M, et al. (2011) Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency. Cell stem cell 8:376-388 Protocol of Directed Reprogramming Profile Detection---qPCR Array Profile Detection---miRNA qPCR