出国研修计划_1.docVIP

出国研修计划 　　Research Plan 　　1. Research title 　　Regulation of lipoxygenase gene expression under low oxygen condition in soybean 　　2. Research background 　　Soybean deterioration and the development of a stale flavor during storage are serious problems that reduce the quality of stored seeds. Fatty acids oxidation is the major cause for the aging in the soybean. The changes of the fatty acids mostly lie on the gene of soybean variety as well as the storage conditions such as temperature, humidity and atmosphere state. 　　Soybean lipoxygenase, greatly consist in mature embryo of the plant seed. Lipoxygenases (EC -) are a family of iron-containing enzymes that catalyse the dioxygenation of polyunsaturated fatty acids in lipids containing a cis, cis-1,4- pentadiene structure. It catalyses the following reaction: fatty acid + O2 = fatty acid hydroperoxide. LOXs are also thought to play an important role in the formation of desirable or undesirable flavor and aroma in many plant products. 　　Low temperature, low O2 or high CO2 was reported to regulate the lox gene expression, especially the low O2 treatment which was able to suppress the accumulation of aging production including the total acid and fatty acid. Therefore, it is necessary to define the lox expression in different soybean varieties stored at the low O2 condition. 　　3. Current research condition 　　The PhD student will be expected to participate in the research project described above but more involved in the study on lox genes in soybean. The student will be mentored by a multidisciplinary science team led by Dr. Sangeeta Dhaubhadel. The other team members include Dr. Yuhai Cui, Dr. Robert Forster and Dr. David from AAFC-PARC. Dr. Dhaubhadel is a senior AAFC research scientist with over 30 years working experience and he has more than 10 years of experience in molecular plant virology, molecular biology and genetic engineering. His research areas have involved in plant breeding, plant tissue culture and molecular