施氮量对烟叶香气物质含量影响及品种间差异研究.docVIP

施氮量对烟叶香气物质含量影响及品种间差异研究 　　摘要 为了弄清烤烟碳氮代谢的品种间差异，以不同施氮量为处理，研究了碳氮代谢关键化合物，分析相关酶的动态变化及其与香气物质形成的关系。结果表明：中烟100和K326在中氮条件下香气物质总量最高，NC89在低氮条件下最高；品种间香气物质总量中烟100最高。碳氮代谢的协调与香气物质总量、各类组分与品种、施氮量的互作有关，其中品种因素起主导作用，烟株前期氮代谢旺盛，后期转向碳代谢的规律有利于香气物质的形成。 　　关键词 施氮量；香气物质；品种；碳氮代谢 　　中图分类号 TS41+1 文献标识码 A 文章编号 1007-5739（2015）18-0019-03 　　Study on Nitrogen Fertilizer Dosage on the Content of Aroma Substances in Tobacco and the Differences of Varieties 　　WU Zu-ju 　　（Shiyan Danjiangkou Tobacco Companies Marketing Department，Danjiangkou Hubei 442700） 　　Abstract In order to understand the difference of carbon and nitrogen metabolism of flue-cured tobacco varieties taking nitrogen application amount as treatment the dynamic change of carbon and nitrogen metabolism key compounds and related enzymes was studied，the relationship between them and aroma matter formation was analyzed.The results showed that total amount of aroma substances of CC100 and K326 were the highest under the middlnitrogen condition，NC89 had the highest gotal amount under low nitrogen condition.Total amount of aroma substances of CC100 was the highest among tobacco varieties.There was the relation between carbon and nitrogen metabolism of coordination，total amount of aroma substances and all and variety and nitrogen application amount of interaction，the varieties played a leading role，nitrogen metabolism in early stage of tobacco plant and later turned to the law of carbon metabolism was conducive to the formation of aroma substances. 　　Key words nitrogen fertilizer dosage；aroma substance；variety；carbon and nitrogen metabolism 　　烤烟碳、氮代谢受遗传基因、环境条件和栽培技术的影响，是一种多基因调控与环境因素交互作用的结果[1]。不同品种由于基因型和遗传基础不同，烟叶碳氮代谢存在很大差异，因此导致化学成分和香气物质含量也不相同。该文通过不同施氮量处理，研究了不同叶龄时期不同品种烟叶在生长发育过程中，碳、氮化合物和相关酶的变化及对烟叶品质的影响，以及品种间碳氮代谢对烟叶各类香气物质形成的相关性差异，为科学施肥、成熟采收以及不同品种制定更加合理的栽培措施提供指导，为优质、特色烟叶生产提供理论依据。 　　1 材料与方法 　　1.1 试验地概况 　　试验于2012年在十堰进行。试验地土壤肥力均匀，地面平整，供试土壤为黄棕壤土，基础土壤肥力为全氮1.21 g/kg，碱解氮69.59 mg/kg，有效磷7.57 mg/kg，速效钾102.43 mg/kg，缓效钾711.58 mg/kg，有机质18.63 g/kg，pH值7.92。