（毕业设计论文）《酸热法提取紫花苜蓿鲜草叶蛋白的工艺参数研究》.docVIP

PAGE 精品 精品 酸热法提取紫花苜蓿鲜草叶蛋白的工艺参数研究 摘 要 苜蓿（Medicago Sataiva L.）是世界上分布最广泛、最古老的豆科栽培牧草之一。苜蓿富含蛋白质、多种维生素和矿物质，是多种家畜喜食的优质蛋白质饲草和能量饲草，它既可用于饲料产业，同时近年也被用来开发研制成各种食品。苜蓿叶片中含有丰富的蛋白质，为一种良好的提取叶蛋白的原材料。 本课题以紫花苜蓿鲜叶为材料，提取方法为酸热法。主要内容是用酸热法提取苜蓿鲜叶中的叶蛋白，本实验通过设置不同的提取温度、pH值、絮凝时间三个单因素进行酸热实验来研究提取率。实验结果表明，酸热法实验各单因素最佳参数分别为：温度为50℃，pH值为4，絮凝时间为10min。并在单因素基础上设计了响应面试验。然后对提取的叶蛋白进行氨基酸含量的测定分析。采取方法：本实验先用打浆机将苜蓿叶打浆，加盐，通过酸热、离心、干燥得到苜蓿叶蛋白，用凯氏定氮法测定不同条件下的蛋白质提取率，并作响应面试验选择最优条件。用选取的最优条件提取叶蛋白，做叶蛋白氨基酸的测定分析。 通过氨基酸分析结果表明,苜蓿叶蛋白氨基酸种类全,含量高,氨基酸组成比例协调, 与成人氨基酸模式基本相符，因此苜蓿叶蛋白为优质蛋白质原。 关键词 苜蓿；叶蛋白；酸热法；提取 Abstract Alfalfa (Medicago Sataiva L.) is one of the worlds most widely distributed and the oldest cultivated forage legume . Alfalfa is rich in protein, vitamins and minerals, is favorite for livestock,a variety of high quality forage protein and energy forage,which it can be used for feed industries, while also being used in recent years develop into a variety of food. Alfalfa leaves are rich in protein, as a good raw materials for leaf protein extraction.  The topic is alfalfa fresh leaves for the materials, samples are extracted with acid heating. Main content is that alfalfa leaf protein is extracted by acid heating, by setting different extraction temperature, pH, flocculation time of three single factor experiments extraction rate is studied the hot acid. The result of the experiment shows that in the single factor acid thermal method experiment ,the best parameters is temperature 50° C，pH=4,coagulation time is 10 minutes. Based on a single factor the response surface experimental is design. Then extracted leaf protein amino acid content is analysised. Methods is that: This study will first use the beater to beat alfalfa leaf, adding salt, by acid, heat, centrifugation, dried alfalfa leaf protein, with the Kjeldahl nitrogen determination method for the determination of protein under different conditions, yield, and test for response surface choose the best conditions. Select the optimum conditions with leaf prote