兔舌癌颈淋巴结转移模型的建立及超声动态检查的试验研究欧阳紫兰.DOCVIP

作者简介：欧阳紫兰，女，硕士生，住院医师，研究方向：口腔颌面外科学。手机邮箱： HYPERLINK mailto:792084916@ 792084916@。 通信作者： 邱丽华，女，手机Email： HYPERLINK mailto:gqyidi2001@163.com gqyidi2001@163.com。 基金项目： 重庆市科学技术研究项目（编号：KJ100322） 兔舌癌颈淋巴结转移模型的建立及超声动态检查的实验研究 欧阳紫兰,邱丽华，王婷，庞谅，曾峥(重庆医科大学附属口腔医院/重庆市口腔疾病与生物医学研究中心,重庆，401147) 【摘要】 目的 用兔肝VX2瘤块接种建立舌癌及颈淋巴结转移的大型动物模型，并于不同时间点进行颈部淋巴结的超声动态检查。 方法 选取新西兰大白兔30只，将VX2瘤块接种于兔舌肌层内，随机分为3组，每组10只,分别于接种第1、3、5周进行颈部淋巴结超声检查，并处死后行舌体肿瘤和颈部淋巴结的大体及病理学检查。结果 接种1周后肿瘤已生长，但未扪及淋巴结肿大，超声检查未见明显的颈部淋巴结影像；3周后可扪及颈部肿大淋巴结，但并未出现转移；5周时剩余的70%实验兔出现颈淋巴结转移，反应性淋巴结与转移淋巴结具有不同的超声影像特点。 结论 兔肝VX2瘤块接种5周后可用于舌癌及颈部淋巴结转移的大型动物模型的实验研究，且超声检查对兔舌癌颈部转移淋巴结有较高的诊断价值。 【关键词】VX2瘤块；动物模型；舌癌；颈淋巴结转移；超声检查 【中图分类号】 R739.86，R73-35+2 Establishment and ultrasonography of the neck lymph node metastasis model of VX2 tongue carcinoma in rabbits Ouyang Zilan, Qiu Lihua, Want Ting, Pang Liang, Zeng Zheng(The Affiliated Hospital of Stomatology of Chongqing Medical University, Chongqing Research Center for Oral Diseases and Biomedical Science，Chongqing ，401147，China) 【ABSTRACT】 Objective To establish the animal model of tongue carcinoma and neck lymph node metastasis in rabbits with intact hepatic VX2 tumor tissue and make ultrasonic testing in different time. Methods The intact VX2 rabbits′ hepatic tumor tissue was transplanted to the lateral border of the tongues of 30 New-Zealand White rabbits divided into 3 groups. Every ten of the rabbits got ultrasonic testing after 1, 3 and 5 weeks and were killed. The specimens of transplanted tongue carcinomas and cervical lymph nodes in the rabbits were examined histopathologically. Results The tumor grew well after 1 week, but no swelling lymph node and ultrasonic image of node. The lymph nodes were swelling after 3 weeks, but no metastases. 70% of the rest rabbits got lymph nodes metastases had different ultrasonic images between the reactive ones. Conclusion The model established with intact VX2 rabbits hepatic tumor tissue is considered to be suitable for tongue canc