响应面法优化乌苏里瓦韦热水提取绿原酸工艺.PDFVIP

中国农学通报 2015,31(22):270-273 Chinese Agricultural Science Bulletin 响应面法优化乌苏里瓦韦热水提取绿原酸工艺 王丽红，姜晓婷，谭凤田 （佳木斯大学药学院，黑龙江佳木斯154007） 摘 要：旨在对乌苏里瓦韦中绿原酸的工艺条件进行优化。采用热水提取乌苏里瓦韦中绿原酸，根据 Box-Benhnken 中心组合设计原理，设计了3 因素3 水平响应面分析试验，来确定乌苏里瓦韦中绿原酸的 最佳工艺条件。结果表明乌苏里瓦韦绿原酸最佳提取工艺条件为：液料比25:1，提取时间4 min ，提取温 度96℃，在这种条件下绿原酸得率可达5.11% ，与理论值相接近，表明响应面分析法优化乌苏里瓦韦中 绿原酸热水提取工艺具有良好的效果。水提法具有能耗少、时间短和低成本等特点，为乌苏里瓦韦提取 绿原酸的工业化生产提供了理论依据。 关键词：乌苏里瓦韦；响应面法；绿原酸 中图分类号：R283.6 文献标志码：A 论文编号：2014-1317 OptimizationofExtractionProcessofChlorogenicAcidfromLepisorusussuriensis(RegeletMaack) ChinginHotWaterbyResponseSurfaceMethodology WangLihong,JiangXiaoting,TanFengtian (CollegeofPharmacy,JiamusiUniversity,JiamusiHeilongjiang154007) Abstract:Theaimwastooptimizethe extractionprocessofchlorogenicacidfromLepisorusussuriensis (Regel etMaack)Ching.ThehotwaterwasusedtoextractchlorogenicacidfromLepisorusussuriensis(RegeletMaack) Ching.AccordingtoBox-Benhnkencentralcompositedesignprinciples,threefactorsandthreelevelsof responsesurfaceanalysistestsweredesignedtodeterminetheoptimum extraction conditionsofchlorogenic acidfromLepisorusussuriensis (RegeletMaack) Ching.Theresultsshowedthatthebestextractionprocessof chlorogenicacidwasasfollows:liquidtomaterialratiowas 25:1,extractiontimewas 4 min,andextraction temperaturewas 96℃.Undersuchcondition,chlorogenicacidyield was upto 5.11% and closetothe theoreticalvalue,indicatingthatthehotwaterextractionprocesshadgoodresultswiththeresponsesurface methodologytooptimizethe extractionprocessofchlorogenicacidfromLepisorusussuriensis (RegeletMaack) Ching.Thewaterextractionhadcharacteristicssuchaslowenergyconsumption,shorttimeandlowcost,which providedtheoreticalbasisforindustriali