绞股蓝黄芪保健茶对免疫功能影响.docVIP

绞股蓝黄芪保健茶对免疫功能影响 　　摘 要：研究绞股蓝黄芪保健茶对小鼠免疫功能的影响。将200只清洁级ICR雌性小鼠，按体重分成免疫5大组，每组随机分成4小组，每小组10只；按人日推荐用量的5倍、10倍、30倍，分别设0.415、0.830、2.490g/kgBw 3个剂量组和蒸馏水对照组。小鼠每天按20mL/kgBW连续灌胃30d后，分别测定小鼠的体重、脏器指数、细胞免疫功能、体液免疫功能、单核-巨噬功能以及NK细胞活性。结果表明：样品高剂量组小鼠淋转OD差值、抗体积数、溶血空斑数和碳廓清吞噬指数a都明显高于对照组，经统计，差异均有统计学意义（p0.05）；高剂量的样品对小鼠的免疫功能有明显的增强作用。 　　关键词：绞股蓝；黄芪；茶叶；免疫力 　　中图分类号 R151 文献标识码 A 文章编号 1007-7731（2018）08-0048-04 　　Effect of Gynostemma Astragalus Health Tea on Immune Function 　　Yang Sufang1 et al. 　　（1College of Pharmacy，Fujian University of Chinese Medicine，Fuzhou 350108，China） 　　Abstract：To study the effect of gynostemma astragalus health tea on immune function in mice. 200 clean grade ICR female mice were divided into 5 groups according to their body weight. Each group was randomly divided into 4 groups with 10 in each group. According to the recommended daily dosage of 5 times，10 times，and 30 times，0.415 ，0.830 and 2.490g/kgBw were set with distilled water control group. The mice were continuously fed with 20 mL/kg BW daily for 30 days. The body weight，organ index，cellular immune function，humoral immune function，monocyte-macrophage function and NK cell activity were measured. The results showed that the OD value，anti-volume number，hemolytic plaque number，and carbon clearance phagocytic index a of the high-dose mice in the high-dose group were significantly higher than those in the control group. Statistically，the differences were statistically significant（p0.05）. High-dose samples have a significant enhancement of immune function in mice. 　　Key words：Gynostemma；Astragalus；Tea；Immunity 　　研究表明，?g股蓝、黄芪富含的多糖、黄酮类、皂苷及茶叶高含量的茶多酚等活性成分都有利于增强机体免疫力，其相关的制剂在免疫治疗中是种很有希望的生物反应调节剂。 　　绞股蓝始载于《救荒本草》，功善益气健脾，化痰止咳，有“七叶参、赛人参、南方人参”等美誉。实验证实，绞股蓝中6种皂苷和人参皂苷相似，能增强免疫力。如绞股蓝总皂苷（Gyp）能提高正常小鼠淋巴细胞的增殖，促进细胞免疫和体液免疫功能[1]，并对环磷酰胺所致小鼠免疫功能低下有明显拮抗作用，对正常小鼠有一定双向免疫调节作用[2]。绞股蓝多糖对小鼠特异性免疫能力有提高作用[3]，且显著增强Heps小鼠NK细胞活性，提高淋巴细胞转化能力及小鼠血清溶血素含量[4-5]。 　　黄芪始载于《神农本草经》，本名“黄耆”，具有益气升阳、固表止汗，利水消肿和托毒生肌等功效，为补药之长，经药理结果可得其对体液免疫、细胞免疫均有促进作用。如黄芪能显著增加血白细胞和多核白细胞的数