变应性鼻炎患者血清中趋化因子ccl17及ccl22的表达及临床意义-耳鼻咽喉-头颈外科专业论文.docx

摘要 摘要 万方数据 万方数据 摘要 目 的 ： 通 过 检 测 胸 腺 活 化 调 节 趋 化 因 子 (human thymus and activation -regulated chemokine,TARC)CCL17 及巨噬细胞源性趋化因子(macrophage-derived chemokine,MDC)CCL22 在变应性鼻炎（allergic rhinitis ，AR）患者血清中的表 达水平，探讨其在变应性鼻炎发病机制中的临床意义。 方法：共选取 60 例实验标本，其中 AR 患者 30 例，非变应性鼻炎（non-allergic rhinitis，NAR）患者 15 例，健康志愿者 15 例。应用酶联免疫吸附试验（enzyme linked immunosorbent assay ，ELISA）法检测标本外周血中趋化因子 CCL17 及 趋化因子 CCL22 表达水平。 结果：①AR、NAR 患者及健康志愿者血清中趋化因子 CCL17 的蛋白含量 均值分别为 673.10±39.11 、611.31±13.51、604.54±12.23。AR 患者与 NAR 患者 及健康志愿者比较差异均具有统计学意义(P 均0.05)； NAR 患者与健康志愿者 两组比较差异无统计学意义(P0.05)。②AR、NAR 患者及健康者血清中趋化因 子 CCL22 的蛋白含量均值分别为 820.62±51.32、722.68±25.99、702.18±22.75。 AR 患者与 NAR 患者及健康志愿者比较差异均具有统计学意义(P 均0.05)； NAR 与健康志愿者两组间比较差异无统计学意义(P0.05)。③AR 患者血清中趋 化因子 CCL17 与 CCL22 的表达水平呈正相关关系（r=0.565,p0.05）。 结论：趋化因子 CCL17 及 CCL22 在 AR 患者血清含量明显升高，且二者呈 正相关，提示其在 AR 发病机制中发挥一定作用。 关键词：变应性鼻炎；胸腺活化调节趋化因子；巨噬细胞源性趋化因子；酶联 免疫吸附测定 II Ab Abstract ABSTRACT Objective: To determine the level of macrophage-derived chemokine(MDC 、 CCL17) and thymus and activation regulated chemokine(TARC、CCL22)in serum of patients with allergic rhinitis(AR), and discuss their role and clinical significance. Methods: 60 cases of specimens is selected，including the patients with 30 cases of allergic rhinitis(AR)15 cases of non-allergic rhinitis（NAR） patients and 15 cases of healthy volunteers. ELISA was applied to assay the chemokines of CCL17 and CCL22. Results : High level of CCL17 and CCL22 was detected in the AR patients, which was significantly different from that of NAR and healthy people. Serum levels of chemokines CCL17 in AR, NAR and healthy subjects was detected for 673.10±39.11 、611.31±13.51、604.54±12.23. Serum levels of chemokines CCL22 in AR, NAR and healthy subjects was detected for 820.62±51.32 、 722.68±25.99 、 702.18±22.75. Concentration of CCL17 and CCL22 in AR were significantly higher than NAR and healthy volunteers , the difference was statistically significant (P0.05).CCL17 showed positive correlation with CCL22 by