变应性鼻炎小鼠模型建立及鼻粘膜水通道蛋白5的表达及意义-外科学(耳鼻咽喉科学)专业论文.docx

林曾萍 林曾萍 遵义医学院硕士学位论文 万方数据 万方数据 变应性鼻炎小鼠模型建立及鼻粘膜水通道蛋白 5 的表达及意 义 中文摘要 目的：1.小鼠变应性鼻炎动物模型的建立及评价。2.探讨水通道蛋白 5 在变应 性鼻炎小鼠鼻粘膜组织中的表达及意义。 方法：1.通过卵清蛋白全身和局部致敏，建立小鼠变应性鼻炎模型，通过行为 学积分对小鼠模型进行评价。ELISA 检测小鼠外周血血清特异性 IgE、IL-4 水平。 HE 染色后显微镜下观察鼻粘膜组织嗜酸性粒细胞等的表达。2.应用实时定量 PCR 检测小鼠鼻粘膜组织中水通道蛋白 5 的 mRNA 的表达。 结果：1.实验组小鼠行为学积分评分均大于 5 分，较对照组明显增高。实验组 外周血特异性 IgE、IL-4 水平较对照组显著增高（P＜0.05），差异有统计学意义。 HE 染色显示实验组小鼠鼻粘膜纤毛大量脱落，组织间质水肿，小血管扩张，大 量嗜酸性粒细胞、嗜碱性粒细胞、浆细胞浸润。2.变应性鼻炎小鼠鼻粘膜组织中 水通道蛋白 5 的 mRNA 含量显著高于正常小鼠（P＜0.05），差异具有统计学意 义。 结论：1、通过卵清蛋白全身致敏及局部激发，成功建立小鼠变应性鼻炎动物模 型。2、变应性鼻炎小鼠鼻粘膜中的水通道蛋白 5 的表达明显增多，提示水通道 蛋白 5 可能参与变应性鼻炎的发病过程，导致鼻腔液体的高分泌。 关键词：变应性鼻炎、水通道蛋白 5、mRNA 2 The Establishment of Model of Mice with Allergic Rhinitis and The Expression and Significance of Aquaporin-5 in Mice Nasal Mucosa with Allergic Rhinitis ABSTRACT Objective:1.To establish and evaluate models of mice with allergic rhinitis. 2.To investigate the expression and significance of aquaporin 5 in mice nasal mucosa with allergic rhinitis . Method:1.The animal models of mice with allergic rhinitis was established by the general and local sensitization with ovalbumin.The animal model with allergic rhinitis was evaluated by behavioral score. The levels of specific IgE and IL-4 in peripheral blood was detected by enzyme-linked immunosorbent assay. The expression of eosinophils was observed in nasal mucosa under the microscope by HE staining. 2.The expression of mRNA of aquaporin-5 in nasal mucosa was detected by RT-PCR. Result:1.Behavior scores of all mice in the experimental group were more than 5 points , significantly higher than the control group. The level of specific IgE and IL-4 of experimental group in peripheral blood was higher than control group.Hematoxylin-eosin staining showed that the cilia in nasal mucosa was off,interstitial edema,vasodilatation and a large number of eosinophil, lymphocytes and plasma cells were soaked. 2.The mRNA of aquaporin-5 in mice nasal mucosa with allergic rhinitis was significantly higher in normal mice. Conclusion:1.The mo