四角蛤蜊提取物抗氧化活性研究--毕业论文设计.docVIP

四角蛤蜊提取物抗氧化活性研究 摘要：关键词：Mactra Veneriformis JI Jing, LIU Rui, WANG Ling-chong, CHEN Shi-yong, WU Hao(, WU Qiu-hui (Nanjing University of Chinese Medicine, Jiangsu Province center for research develop of marine drug, Nanjing 210046, China) Abstract：Objective: Tracking the activity though preliminary separation, we can figure out the chief substances of Mactra Veneriformis in antioxidant. Methods: According to the process line of water extraction and alcohol precipitation, total clam extract (MV), alcohol precipitation (MP) as well as supernatant (MS) have been got. Except the control group, all the other groups were subjected to subcutaneous injection of D-galactose solution. Mice were treated with three samples once a day for the creation of them low and high-dose groups. The activities of SOD and GSH-PX, CAT and the content of MDA in serum and liver were determined after a trial period of 6 weeks. Four sections were separated form competitive part and verified the activity of scavenging free radical, chelating ability of ion and reducing power. Results: The activities of SOD, GSH-PX and CAT exhibited a significant increase(P＜0.05), the activities of MDA also decrease in liver (P＜0.01) due to Mactra Veneriformis treatment. The activity of GSH-PX and SOD in serum exhibited an obvious increase(P＜0.05); meanwhile, an obvious decrease of MDA activity in serum was also observed(P＜0.05). Furthermore, extracts of MS exhibited the scavenging efficacy of O2- and DPPH，in which the water extracts showed the significant effect, while acetic ether extracts showed optimum chelating ability. Conclusion: Mactra Veneriformis performances antioxidant activity in experiments in vivo and vitro. The efficacious substances are in the water extracts part of MS. Key words：Mactra Veneriformis；supernatant；antioxidant 前言海洋软体动物四角蛤蜊Mactra?Veneriformis Reeve），属瓣鳃纲，蛤蜊科，是我国沿海常见的潮间带经济贝类传统中医理论认为蛤蜊性寒、味咸、无毒，归胃、肝、膀胱经，有滋阴、利水、化痰、软坚之功用，主治消渴、水肿、痰积等。[2]。然而关于四角蛤蜊的体内抗氧化效果鲜有报道。 本文以背部注射D-半乳糖方法建立的小鼠衰老模型