玉米茎基腐病生防菌的筛选及应用-微生物学报-中国科学院微生物.PDF

微生物学通报 Oct. 20, 2017, 44(10): 2345−2352 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.170336 研究报告 玉米茎基腐病生防菌的筛选及应用 张思奇 孙丽萍 赵同雪 徐澜坤 耿肖兵 李永刚* (东北农业大学农学院 黑龙江省高校寒地作物品种改良与生理生态重点实验室 黑龙江 哈尔滨 150030) 摘 要：【目的】拟针对禾谷镰孢进行生防菌的筛选和应用研究，以期为玉米茎基腐病生防菌 剂的研制奠定基础。【方法】通过对峙培养法对玉米茎基腐病的主要致病菌禾谷镰孢进行玉米 内生生防细菌的筛选，从玉米主栽品种九单48 幼苗内部获得一株具有较强抑菌效果的内生生 防细菌(简称48SJ7-1)；基于传统鉴定、16S rRNA 基因序列测定及系统发育分析，对 48SJ7-1 进行鉴定；并通过盆栽试验测定该生防菌株的防效。【结果】菌株48SJ7-1 经鉴定为甲基营养型 芽孢杆菌，GenBank 登录号为KU377993 ，48SJ7-1 盆栽防效为68.47% ，与对照药剂2%戊唑醇 悬浮种衣剂差异不显著。【结论】48SJ7-1 对玉米茎基腐病的主要致病菌禾谷镰孢有较好防效， 对玉米生长还有明显的促进作用，而且表观上无药害发生。 关键词：玉米茎基腐病，拮抗菌，禾谷镰孢，筛选，防效 Screening and application of bio-control bacterium against corn stalk rot caused by Fusarium graminearum ZHANG Si-Qi SUN Li-Ping ZHAO Tong-Xue XU Lan-Kun * GENG Xiao-Bing LI Yong-Gang (Key Laboratory of Cold Crop Breed Improvement and Physiological Ecology in Heilongjiang Province, Agricultural College, Northeast Agricultural University, Harbin, Heilongjiang 150030, China) Abstract: [Objective] To study the screening and application antagonisms of Fusarium graminearum in order to lay the foundation for use of bio-control agents for corn stalk rot. [Methods] The antagonism of F . graminearum were screened from corn endophytic bacteria using the confrontation culture method. A bacterial strain (named as 48SJ7-1) which showed inhibition to F . graminearum was obtained. The bacterial strain was ident