高效石油降解菌的筛选及其降解特性.PDFVIP

业环境科学学报 园园8熏 7穴1雪:0230-0233 允燥怎则灶葬造 燥枣 粤早则燥鄄耘灶增蚤则燥灶皂藻灶贼 杂糟蚤藻灶糟藻 高效石油降解菌的筛选及其降解特性 1 2 2 2 杨雪莲 袁李凤梅 袁刘婉婷 袁李 刚 (1. 沈阳大学环境工程重点实验室袁辽宁 沈阳 110044 曰2. 中国科学院沈阳应用生态研究所袁辽宁 沈阳 110016冤 要院从辽河油田和大庆油田石油污染土壤中分离筛选出两株高效石油降解菌L 和D 菌株袁经形态观察尧生理生化反应袁确定 10 6 此两株菌分别为芽孢杆菌属中的枯草芽孢杆菌渊Bacillus subtilis 冤和地衣芽孢杆菌渊Bac illus licheniformis 冤遥 采用室内盆栽培养方法袁 研究了石油烃的浓度和性质对两菌株降解活性的影响遥结果发现袁土壤中石油烃的含量和处理时间均影响微生物的降解效果袁在处 理 10 d 时袁石油烃的去除率随 污染强度的增加而降低曰随 处理时间的延长袁微生物适应环境后袁在石油烃含量为0.5%~2.0% 时袁石油烃的去除率随 浓度的增加而升高袁在石油烃含量为2.0%~10.0%时袁石油烃的去除率随 浓度的增加而降低曰石油烃的性 质影响菌株的生物活性袁L 和D 两菌株对稀油的去除效果明显高于对稠油的去除效果袁 各组分的去除率依次为烷烃跃芳烃跃胶质 10 6 沥青质袁两菌株对不同性质的石油烃中的烷烃尧芳烃和胶质沥青质的去除率不同遥 ： 关键词 石油降解菌；筛选；降解特性；去除率 中图分类号院X172 文献标识码院粤 文章编号院1672-2043(2008)01-0230-04 Isolation of Petroleum-degrading Strains and Their Degrading Characteristics 1 2 2 2 YANG Xue-lian , LI Feng-mei , LIU Wan-ting , LI Gang (1. Shenyang Key Laboratory of Environmental Engineering, Shenyang University, Shenyang 110044, China; 2. Institute of Applied Ecology, CAS, Shenyang 110016, China) Abstract 院Petroleum -degrading strains L10 and D6 were obtained by selective enrichment culture from eight contaminated soil samples col原 lected from Liaohe and Daqing Oil Fields. The tests in shaking flasks showed that they could degrade petroleum hydrocarbon well and the re原 moval rates could reach 39.5% and 47.9% at 30 d. According to morphologic observation, physiological and biochemical characteristics, they were identified as Bac illus s ubtilis and Bacillus licheniformis re