《色谱峰成分识别技术用于苏丹红检测的研究》毕业学术论文.docVIP

PAGE l 河南科技学院 本科毕业论文 论文题目 色谱峰成分识别技术用于 苏丹红检测的研究 学生姓名：××× 所在院系：食品学院 所学专业：食品科学与工程 导师姓名：××× 完成时间： 年6月1日 摘 要 研究了二极管阵列高效液相色谱仪检测苏丹红的成分识别技术，利用三维图谱对峰的纯度以及峰的组成进行手工和自动确认。在优化的色谱条件下，苏丹红1、2、3及4号得到较好分离，利用峰的前中后部光谱的一致性确认峰的纯度，通过与标准光谱图库的匹配，在保留时间一致的基础上，对样品中的苏丹红组分峰进行进一步的确认，避免假阳性的判断。经过对辣椒粉的检测，取得了满意效果。 关键词：高效液相色谱；苏丹红；识别技术 The research on the chromatograph peak ingredient recognition technology applied in the examination of Sudan red School of Food Science Huihui Chen Abstract The research has studied recognition technology regarding the diode array high performance liquid chromatography applied in the examination Sudan red ingredient, and carried on the handwork and automatic confirmation to the peak purity as well as the peak complsiton by means of the three dimensional atlas. Under the optimized chromatograph condition, the Sudan red 1, 2, 3 and 4 obtain well separates, using the peak front behind spectrum uniform confirmation peak purity, through with the standard spectrum map storage match, in the retention time consistent foundation, carries on the further confirmation to in the sample Sudan red component peak, avoids the false positive judgment. Passes through to the chili powder examination, has obtained the satisfactory effect. Key words：high performance liquid chromatography；Sudan red；recognition technology 目 录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc198964150 引言 PAGEREF _Toc198964150 \h 1 HYPERLINK \l _Toc198964151 1 材料与方法 PAGEREF _Toc198964151 \h 2 HYPERLINK \l _Toc198964152 1.1 材料 PAGEREF _Toc198964152 \h 2 HYPERLINK \l _Toc198964153 1.2 试验方法 PAGEREF _Toc198964153 \h 2 HYPERLINK \l _Toc198964154 1.2.1 仪器与设备 PAGEREF _Toc198964154 \h 2 HYPERLINK \l _Toc198964155 1.2.2 试剂与标准品 PAGEREF _Toc198964155 \h 2 HYPERLINK \l _Toc198964156 1.2.3 样品的处理 PAGEREF _Toc198964156 \h 2 HYPERLINK \l _Toc198964157 1.2.4 色谱条件 PAGEREF _Toc198964157 \h 2 HYPERLINK \l _Toc198964158 1.3 标准品准备 PAGEREF _Toc198964158 \h 2 HYPERLINK \l _Toc198964159 2 结果与分析 PAGEREF _Toc1989641