陆地棉黄萎病抗性的分子标记辅助选择效果-中国农业科学院棉花研究所.PDF

花 学 报 Cotton Science 20 10 22 渊6 冤院527~532 地棉黄萎病抗性的分子标记辅助选择效果 * 祥瑞 王红梅 陈 伟 赵云雷 李运海 龚海燕 桑晓慧 中国农业科学院棉花研究所/ 农业部棉花遗传改良重点实验室袁河南安阳455000 冤 摘要院采用分子标记辅助选择技术将与陆地棉黄萎病抗性相关的18 个SSR 标记用于大田辅助选择育种遥 结 果表明袁18 个标记中的BNL1721尧BNL2733 和BNL3452 标记在有/ 无标记基因型个体间病指差异显著袁且选 择效应能够在不同世代间稳定遗传遥 通过对单标记尧多标记选择效应比较袁发现不同标记间配制辅助选择最优 组合可以实现选择效应的 大叠加遥 关键词院陆地棉曰黄萎病曰分子标记辅助选择曰SSR 中图分类号院S562:Q341 文献标识码院A 文章编号院1002-7807(2010)06-0527-06 * KONG Xiang-rui, WANG Hong-mei , CHEN Wei, ZHAO Yun-lei, LI Yun-hai, GONG Hai-yan, SANG Xi- ao-hui ( 455000, ) To aim at improving the selective efficiency of wilt resistance in upland cotton breeding, the molecular marker assisted selection is used. 18 SSR markers related to Verticillium wilt resistance have been chosen. Among them, three markers, BNL1721尧BNL2733 and BNL3452, presented remarkable differential disease index between markered and non-mark- ered genotypes. Research showed that the selective effect could stably inherited in different generations. After comparing single marker and multiple markers assisted efficiency, we have come to the conclution that use different markers to make optimal combination can maximize the synergy selective efficiency. upland cotton; wilt disease; molecular marker assisted selection; SSR 由于陆地 ( L.)缺乏优 复系的培育 成功地获得了带有恢复基因 的