宫颈癌患者放疗前后乳酸菌的变化及药敏分析-实用预防医学.PDF

宫颈癌患者放疗前后乳酸菌的变化及药敏分析 1 2 3* 3 3 张彩霞 ；苟占彪 ；车团结 ；张莹 ；冯海霞 1. 兰州大学第一医院妇产科，甘肃，兰州，730000;2. 甘肃省中医院重症医学科，甘肃，兰州，730050;3. 甘肃省 生物芯片工程实验室，甘肃，兰州，730000 摘要: 目的：调查妇科宫颈癌患者术后放疗前后，阴道分泌物中乳酸菌的分布情况及对4种抗生素的耐药性，为预防 和治疗继发感染提供实验依据。方法：采用细菌培养法，对阴道分泌物标本中乳酸菌进行检测，并进行药敏实验， 分析放疗前后乳酸菌检测结果的差异性。结果： 放疗前乳酸菌的检出率为76.98%，放疗后乳酸菌检出率45.24% ， 差异有统计学意义（p ＜0.05 ）。乳酸菌对甲硝唑、庆大霉素、阿米卡星、头孢噻肟钠的体外抑菌效果：头孢噻肟 钠耐药性最低，其他依次是阿米卡星、庆大霉素、甲硝唑，耐药率分别为4.7% 、5.8%、76.8%、84.5%。结论：宫 颈癌术后放疗可影响阴道的微生态平衡，这可能是导致宫颈癌术后放疗患者发生感染的重要原因之一；由于乳酸 菌对甲硝唑的耐药率最高，可认为在预防放疗后的阴道炎感染时，甲硝唑对阴道益生菌的影响不大，应作为首选 药。乳酸菌对头孢噻肟钠最敏感，临床在治疗阴道感染时，需要注意避免使用该药物。 关键词:宫颈癌；放疗；乳酸菌；抗生素；耐药性 Study the changing of lactic acid bacteria and drug sensitivity analysis pre and post che motherapy in patients with cervical cancer 1 2 3* 3 3 ZHANG Cai-xia ,GOU Zhan-biao ,CHE Tuan-jie ,ZHANG Ying ,FENG Hai-xia , Lanzhou university s First Hospital,Lanzhou 730000,china Abstract:Objective ：In this study, we investigated the existance of lactobacillus in the vaginal discharge of the postoperative cervical cancer patients before-and-after radiation therapy , and the resistance of lactobacillus to 4 antibiotics, to provide evidences for preventing and treating the secondary infections after operation fcervical cancer. Methods: A germiculture method was employed. Lactobacillus from the vaginal discharge samples of the cervical cancer patients before and after radiation therapy were cultured for statistic and drug sensitive test. Results: The detection rate of lactobacillus before radiation therapy was 76.98%, higher than that after radiation therapy (45.24%), The result was stat