转基因鱼腥藻7120适宜生长条件的初步研究.PDF

2003, 21(5) : 406410 J ournal of Wuha n Botanica l Resear ch 7120 1, 2, 3 3 2 1 欧阳叶新 , 施定基 , 梁承邺 , 胡鸿钧 ( 1. / , 430074; 2. , 510650; 3. , 100093) : (T NF-) 7120 , TNF- 7120 ,7120 25 30 , pH 7.07. 5, 7120 - 2 - 1 , 10700 ( 4 / ) E m s g L 7120 7120 T NF-: 8 d 7120 T NF-, 7120 : - 7120; ; ; ; ; TNF pH : 945.3: : 1000-470 (2003) 05-0406-05 Q A Preliminary Studies on Optimal Growth Conditions for Transgenic Microalgae Anabaena sp. PCC7120 1, 2, 3 1 2 3 OUYANG Ye- in , SHI Ding-Ji , LIANG Cheng-Ye , HU Hong-Jun ( 1. Wuhan Botanical Garden Wuhan Institute of Botany The Chinese A cademy of Sciences Wuhan/ , , 430074, China; 2.South ChinaInstitute of Botany The ChineseA cademy of Sciences Guangzhou, , 510650, China; 3.Institute of Botany , The Chinese A cademy of Sciences, Beijing100093, China) A stract: Our previous work presented the construction of transgenic microalgae Anabaena sp. PCC7120 (A . 7120) with human tumor necrosis factor-(hTNF-) gene. We currently described the preliminary studies on optimal growth conditions for transgenic A . 7120 in the laboratory- scale. T he data we provided here showed that the optimal growth temperature and pH are at 25 30 , and pH 7. 0 7. 5, respectively; the net photosynthetic oxygen elution rates of the trans- genic A . 7120 under different photon flux densities measured here