耐受高体积分数丁醇乳酸菌的筛选及其鉴定.PDFVIP

212 2013, Vol.34, No.03 食品科学 ※生物工程 耐受高体积分数丁醇乳酸菌的筛选及其鉴定 1 1,2,* 2 1 1 刘洪儒 ，曹龙奎 ，孙大庆 ，宋 亮 ，杨春宇 (1.黑龙江八一农垦大学食品学院，黑龙江 大庆 163319 ； 2.黑龙江省农产品加工工程技术研究中心，黑龙江 大庆 163319) 摘 要：从不同样品中分离和筛选在较高丁醇体积分数下生长的菌株，通过不断提高培养基中的丁醇添加量测定 菌株的相对生长率，经过初测和复测，选择相对生长率较高的菌株绘制其耐受曲线，分析其能够耐受丁醇的体积 分数。对菌株进行常规生理生化鉴定和16S rDNA序列分析，结合菌体形态及菌落特征确立菌株的种属。筛选得到2 株耐受丁醇的体积分数均达到了3%的菌株，分别为A37和A50 。经鉴定，序列分析与生理生化实验结果是一致的， A37为Enterococcus faecalis ，A50为Lactobacillus fermentum 。结果表明两株菌具有较高的丁醇耐受性。 关键词：乳酸菌；丁醇耐受性；相对生长率；筛选；鉴定 Screening and Identification of Butanol-Tolerant Lactic Acid Bacteria 1 1,2,* 2 1 1 LIU Hong-ru ，CAO Long-kui ，SUN Da-qing ，SONG Liang ，YANG Chun-yu (1. College of Food Science, Heilongj iang Bayi Agricultural University, Daqing 163319, China ； 2. Agri-food Processing and Engineering Technology Research Center of Heilongj iang Province, Daqing 163319, China) Abstract ：Bacterial strains that can grow well in the presence of butanol at high concentration were isolated and screened from different samples. Their relative growth rates in the medium with the addition of butanol were monitored. Subj ected to repeated screening, the strains with higher relative growth rates were chosen to make the butanol-resistant curve. Meanwhile, the species of the selected strains were identified by physiological and biochemical experiments and 16S rDNA analysis coupled with bacterial colony characteristics. Two bacterial strains (A37 and A50) revealed