苏云金芽胞杆菌标记重组菌株的构建与杀虫基因水平转移-高电压技术.PDFVIP

200 1 16 1 CHINESE JOURNAL OF APPLIED ECOLOGY, Jan. 200 , 16( 1) %142~ 146 * * * 周 琴 孙 明 李 林 杨在清 喻子牛 ( , 430070) SOE gf p cry 1A c 10 pAD44 12 pBMBZGC10, CryB CryB(pBMBZGC10) . CryB( pBMBZGC10) 7 8 - 1 3060 90 ml 3 , 10 ~ 10 !ml , , , cry 1A c 10 , . ( gf p ) 1001- 9332(200 ) 01- 0142- 0 Q784 A Construction of Bacillus thuringiensis labeled recombinant strain and horizontal transfer of its cry 1A C10 gene. ZHOU Qin, SUN Ming, LI Lin, YANG Zaiqing, YU Ziniu ( State Laboratory of A gricultural M icrobiolo gy, H uaz hong A gricultural University, Wuhan 430070, China) . Chin . . App l . Ecol . , 200 , 16( 1) : 142~ 146. A recombinant plasmid pBMBZGC10 w as obtained by the ligation of gf pcry 1A c 10 fusion gene and vector plas mid pAD44 12, w hich w as then introduced by gene pulser into acrystalliferous strain CryB, and a recombinant strain CryB( pBMBZGC10) was obtained. Different fermentative solutions of recombinant strain were used for mult i spraying on Brassica p ekinesis, Ip omoea aquatica and L ycop ersicon esculentum leaves. The results of fluo rescent detection and PCR amplification revealed that cry 1A c 10 gene did not transfer into indigenous bacteria, actinomyces and fungi in test soil, and could not be detected in roots, stems and leaves of test plants. Key words Bacillus thuringiensis , Green fluorescent gene gf p , Insect icide crystal gene protein gene cry , Horizontal gene transfer . . , 1 ,