长双歧杆菌BBMN68微胶囊的制备及其应用性评价.PDF

※生物工程 食品科学 2015, Vol.36, No.09 71 长双歧杆菌BBMN68微胶囊的制备及其 应用性评价 杨剀舟，刘 云，杨立芳，冷小京* （中国农业大学食品科学与营养工程学院，北京 100083 ） 摘  要：长双歧杆菌BBMN68分离自广西巴马长寿老人粪便，已证明其具有双歧杆菌普遍的生理功能。为解决其 不耐酸、不耐氧的缺点，本实验采用微胶囊包埋技术，以海藻酸钠、乳清蛋白以及VE为壁材，在氮气体系中采用 挤压法包埋该双歧杆菌活菌体制备湿微胶囊和冻干微胶囊。结果表明：海藻酸钠、乳清蛋白和VE质量分数分别为 2%、3%和10%条件下制备的湿微胶囊在酸奶中保存21 d仍能保持106 CFU/g 以上的活菌数，耐胃酸实验2.0 h时活菌 7 CFU/g ，肠溶实验中微胶囊在2.0 h实现完全崩解；海藻酸钠和乳清蛋白质量分数为2%和3%，氮 数仍高达3.16 ×10 气环境下制备的冻干微胶囊通过恒温加速实验测定可在室温条件下贮藏长达75 d 。通过微胶囊包埋技术较好地解决 了长双歧杆菌BBMN68不耐酸、不耐氧的缺点，同时也提供一种新型有效的补充肠道益生菌的形式。 关键词：长双歧杆菌BBMN68 ；双乳化；微胶囊；真空冷冻干燥；模拟胃肠实验 Preparation and Application of Bifidobacterium longum BBMN68 Microcapsules * YANG Kaizhou, LIU Yun, YANG Lifang, LENG Xiaojing (College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) Abstract: Bifidobacterium longum subsp. longum BBMN68 is a new strain isolated from the feces of a centenarian living in an area of Bama, Guangxi, China, known for the longevity of its residents. BBMN68 has general health benefits of Bifidobacterium, but it is sensitive to oxygen and low pH. In order to resolve the shortcomings, microcapsules were prepared by using sodium alginate, whey protein and vitamin E as wall materials, an extrusion method was used to protect BBMN68 cells in the nitrogen environment, and freeze-dried microcapsules were produced simultaneously. The results showed that the wet microcapsules prepared under the conditions of sodium alginate 2%, whey protein 3%, vitamin E 10% (wt%) could 6 7