水稻三明显性核不育基因的定位.PDF

HEREDITAS (Beijing) 2012 5 , 34(5): 615―620 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2012.00615 水稻“三明”显性核不育基因的定位 1 2 3 1 1 2 2 1,2 杨泽茂 , 谢小芳 , 黄显波 , 王丰青 , 童治军 , 段远霖 , 兰涛 , 吴为人 1. , 310000; 2. , 350002; 3. , 365509 “三明显性核不育水稻”突变体是由福建省三明市农业科学研究所于2001 年在杂交组合 “SE21S/Basmati370”的F2代群体中发现的。其不育性受 1 个显性基因控制(将该基因命名为SMS) 。经过多代回 交, 该显性不育基因已导入籼稻品种佳福占的遗传背景中(将该不育材料称为佳不育) 。为了定位SMS, 文章将佳 不育与粳稻品种日本晴杂交, 并将F1与佳福占测交, 构建了一个作图群体。利用SSR和INDEL标记, 通过混合分 离分析和连锁分析, 将SMS定位于第8 号染色体上两个INDEL标记ZM30 和ZM9 之间, 约99 kb 的区间内。该结 果为克隆SMS 奠定了基础。 水稻; 显性雄性核不育; 基因定位 Mapping of Sanming dominant genic male sterility gene in rice 1 2 3 1 YANG Ze-Mao , XIE Xiao-Fang , HUANG Xian-Bo , WANG Feng-Qing , TONG Zhi-Jun1 2 2 1,2 , DUAN Yuan-Ling , LAN Tao , WU Wei-Ren 1. College of Agriculture Biotechnology, Zhejiang University, Hangzhou 310000, China; 2. College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3. SanMing Institute of Agricultural Science, Shaxian 365509, China Abstract: The mutant of “Sanming Dominant Genic Male Sterile Rice” was found from an F2 population of cross “SE2lS/Basmati370” by Sanming Institute of Agricultural Science in 2001. It has proven that the male sterility of this mu- tant is controlled by a dominant gene (named as SMS). By multiple backcrosses, this dominant male sterile allele was intro- duced into the genetic backgr