顶空固相微萃取与气质联用法分析-上海海洋大学学报.PDFVIP

第 14 卷第 2 期 上海水产大学学报 Vol. l4 , No.2 JOURNAL OF SHANGHAI FISHERlES UNIVERSfIY June ，2:∞5 2∞5 年 6 月 文章编号: 1004 - 727 1(2005)02 - 0176 - 05 顶空固相微萃取与气质联用法分析 链肉中风昧成分 王锡昌，陈俊卿 (上海水产大学食品学院，上海 2删90) 摘 要:以我国重要食用的淡水鱼之一，链为原料。采用聚二甲基硅氧皖-二乙烯苯(PDMS- DVB) 涂层的固相 微萃取头萃取鱼肉中的挥发性成分，并通过气质联用仪分析鉴定鱼肉中的气味成分。结果表明:固相微萃取 技术有效地吸附了鱼肉中的挥发性成分，经 NIST 质谱数据库检索和文献对照，共检出并确定 48 种成分，所检 出物质的分子量范围大部分处于90- 250 MW 之间，并且这些成分多是一些激基类及醇类化合物，其总百分 含量高达 88.23% ，其中以 1-辛烯-3 醇的百分含量最多，为 18.950毛， 1-辛烯-3 醇一般表现为土昧、蘑菇味。其 次是一些直链的饱和睦类，如己腔、庚醒、辛醒、圭酶、葵醒等，而这些睦类通常产生一些令人不愉快、辛辣的刺 激性气味，并且普遍存在于淡水鱼中O 关键词:顶空 固相微萃取;气相色谱质谱;挥发性成分;链 中图分类号:TS 201.2 文献标识码:A Analysis of flavors in silver carp meat by headspace solid phase microextraction combined with GC-MS WANG Xi-chang , CHEN Jun叮mg ( College of Food Science and Techrwlogy , Shanghai Fisheri臼 University ， Shanghai 2仅lO90 , China) Abs位act: Silver carp , Hypophthαlmichthys molitrix meat was selected as material , and its odor components was extracted and determined by headspace solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) , meanwhile the SPME fiber was coated with polydimesiloxane-divinylbenzene (PDMS- DVB). The results showed that SPME could be effective in volatiles analysis of silver carp meat , and 48 volatile components were identified from 49 compounds by comparing their mass spectra with those contained in the NIST mass spectral database ,the molecular weight of most of these compounds in