基于纳米金显色的非标记核酸适配体可视化检测重金属镉的方法研究.PDF

7 2 Vol. 7 No. 2 2016 2 Journal of Food Safety and Quality Feb. , 2016 * 祎 栾云霞 , 张展宁, 陈佳 , 徐 笠, 陆安祥 (, (), , 100097) 摘 要: 目的 方法 , , DNA , , 结果 0.14~10 ng/mL 0.14 ng/mL, , 结论 , , 关键词: ; ; ; ; Visual detection of cadmium based on aggregation of aptamer-modified gold nanoparticles * LUAN Yun-Xia , ZHANG Zhan-Ning, CHEN Jia-Yi, XU Li, LU An-Xiang (Beijing Research Center for Agricultural Standards and Testing, Risk Assessment Lab for Agro-products (Beijing), Ministry of Agriculture , Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing 100097, China) ABSTRACT: Objective To establish a visual, rapid, and sensitive method for the detection of Cd based on aptamer and gold nanoparticles. Methods The concentration of cadmium ion was detected indirect by the value of absorbance according to the color changes caused by salt-induced condensation of gold nanoparticles and the high affinity binding of aptamer with cadmium. By optimizing the concentration of aptamers DNA, salt and gold nanoparticles in solution, the optimal determ