甘蓝未受精子房离体培养及再生植株倍性的鉴定-中国农学通报.PDF

中国农学通报 2017,33(18):42-48 Chinese Agricultural Science Bulletin 甘蓝未受精子房离体培养及再生植株倍性的鉴定 贾思齐，王 超，王 帅，贾学方 （东北农业大学园艺学院，哈尔滨 150030） 摘 要：甘蓝通过未受精子房离体培养诱导获得的再生植株，对再生植株的倍性进行有效的鉴定是将其 进一步应用于优良品种选育的基础。本研究利用3 种基因型的甘蓝材料(PMQM、QMF、RMQM)培育再 生植株，优化甘蓝未受精子房离体培养体系，并通过形态学鉴定法、根尖染色体计数法、流式细胞仪鉴定 法对组培植株进行倍性鉴定。结果表明：在0.4 mg/L ZT 的分化培养基中，3 种基因型材料的愈伤组织分 化率明显高于1.0 mg/L 6-BA 培养基中的组培苗，其中基因型RMQM 的分化效果最好；最终确定诱导愈 伤组织分化不定芽的最适培养基配方为MS+0.4 mg/L ZT+ 2.0 mg/L 2,4-D+0.1 mg/L NAA ，且通过3 种 鉴定方法，得出再生植株倍性：单倍体3.4% ，双倍体49.8% ，四倍体15.9% ，嵌合体35.3%。 关键词：甘蓝；未受精子房；离体培养；再生植株；倍性鉴定 中图分类号：S635.1 文献标志码：A 论文编号：cas Cabbage:CultivationofUnfertilizedOvaryinVitroandPloidyIdentification ofRegeneratedPlant JiaSiqi,WangChao,WangShuai,JiaXuefang (CollegeofHorticulture,NortheastAgriculturalUniversity,Harbin150030) Abstract:Theregeneratedplantofcabbagewasobtainedbycultivatingitsunfertilizedovaryinvitro,effective ploidyidentificationofregeneratedplantwouldlayasolidfoundationforitsfurtherapplicationinbreedingand selectingexcellentvarietiesofcabbage.Inthisstudy,threedifferent-genotypesof cabbage(PMQM,QMF, RMQM)werechosentoproducenewregeneratedplants,improvetheculturesystemofunfertilizedovaryof cabbage in vitro, and identify the ploidy of regenerated plant by using morphological identification, chromosomecountinginroot-tipcells,andDNAflowcytometry.Theresultsshowedthatthedifferentiation ratesofcallusofthreegenotypescultivatedindifferentiationmediumcontained0.4mg/LZTweresignificantly higherthanthatinthemediumwith 1.0mg/L6-BA,theRMQMgenotypedemonstratedthebestdifferentiation efficiency.Finally,themediumMS+0.4mg/LZT+2.0mg/L2,4-D+0.1mg/LNAAwasidentifiedasthemost suitablemediumforthedifferentiationofadventitio