单宁酸对高原鼠兔和根田鼠生殖激素的影响-兽类学报.PDF

兽类学报,2011,31 (3):278 - 283 Acta Theriologica Sinica The effect of tannic acid on the gonad hormones in plateau pikas (Ochotona curzoniae )and root voles (Microtus oeconomus )  DAI Xin,ZOU Zhaozhi,ZHANG Tingting,JIANG Wei,WEI Wanhong,YANG Shengmei (College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009 ,China) Abstract :Plateau pikas (Ochotona curzoniae)and root voles (Microtus oeconomus)are herbivorous mammals inhabiting in the highest plateau of the world,the Qinghai-Tibet Plateau They feed on plants that contain many kinds of secondary metabolites. Our study objectives were to verify that secondary metabolites in their diets have some effects on the hormones related to their reproductive behaviors,and to explore the co-evolution between the animals and their forage for both spe- cies. Gonadotropin-releasing hormone (GnRH),plasma androgen and estrogen for both species was assayed after subjects were administrated tannic acid at dosages of 0 ,5,10,20 mg/ kg BW for 1,3,or 5 days. Tannic acid increased plasma androgen and estrogen in both of pikas and root voles. We observed no significant change of GnRH in either species. This suggests that tannic acid plays a role in sexual maturation and benefits reproductive behaviors Key words :Coevolution ;Gonad hormone;Plateau pikas (Ochotona curzoniae ); Root voles (Microtus oeconomus ); Tannic acid 单宁酸对高原鼠兔和根田鼠生殖激素的影响 戴鑫 邹昭枝 张婷婷 蒋巍 魏万红 杨生妹 (扬州大学生物科学与技术学院,扬州 225009 ) 摘要:高原鼠兔和根田鼠是生存于青藏高原的小哺乳动物,它们所取食的高原植物含有多种次生化合物。为了 证明食物中的次生化合物对高原鼠兔和根田鼠繁殖相关激素的影响,探讨它们与取食植物间的协同进化,分别 对两种动物灌服0、5、10 和20 mg/ kg BW 剂量单宁酸 1 d、3 d 及 5 d 后,测定其下丘脑促性腺