高效液相色谱法测定食品中糖醇.PDFVIP

278 2007, Vol. 28, No. 06 食品科学 ※分析检测 高效液相色谱法测定食品中的糖醇 1 1 1 1 2 2 2 李 黎 ，刘玉峰 ，唐华澄 ，李 东 ，王 晶 ，方 向 ，吴方迪 (1.北京市营养源研究所分析室，北京 100069；2.国家标准物质研究中心，北京 100013) 摘 要：采用高效液相色谱法对食品中的木糖醇、山梨糖醇和麦芽糖醇进行了定量测定研究，最终确定的最 佳分析条件为 Waters NH2 氨基色谱柱；示差折光检测器 40℃柱温；流动相：乙腈:水 85:15(V/V) ；流速： 1.0ml/min；进样量：10μl。该方法的精密度RSD＜2%，回收率大于98%。在此色谱条件下20min内完成了糖醇 的分离，结果比较理想。研究表明此新建方法准确而快速，并能适用于绝大部分食品中这三种糖醇的测定。 关键词：高效液相色谱；木糖醇；山梨糖醇；甘露糖醇；食品 Determination of Sugar Alcohol in Foods by HPLC Method 1 1 1 1 2 2 2 LI Li ，LIU Yu-feng ，TANG Hua-cheng ，LI Dong ，WANG Jing ，FANG Xiang ，WU Fang-di (1.Laboratory Department of Beijing Research Institute for Nutritional Resources, Beijing 100069, China； 2.National Research Center for Standard Substances, Beijing 100013, China) Abstract ：A HPLC method was established to determine xylitol, sorbitol and maltitol in foods. The final chromatographic conditions are as follows: using amino chromatographic column of waters NH2 with refractive index detector, elution influent is 85:15 methane:water (V/V), flow rate 1.0 ml/min and injection volume 10μl . The repeatability relative standard deviations of this method is below 2% and the recovery is over 98%. A satisfied result is obtained under this chromatographic conditions, and the whole separation can be finished within 20 min. The results showed that this new HPLC analysis method is both correct and fast enough to be used to determine most kinds of foods containing these three sorts of sugar alcohols. Key words：HPLC；xylitol；sorbitol；mannitol；foods 中图分类号：O657.72 文献标识码：A 文章编