阿托伐他汀对糖尿病大鼠急性心肌梗死后心功能及hgfc-met信号通路的影响-心血管专业论文.docxVIP

暨南大学 暨南大学硕士学位论文 阿托伐他汀对糖尿病大鼠 AMI 后心功能及 HGF/c-Met 信号通路的影响 PAGE PAGE IV Abstract Objective relevant clinical and experimental studies have showed that the ventricular remodeling and cardiac function deterioration of the diabetic with AMI were more apparent than non-diabetes. This study was to investigate effect of atorvastatin on myocardial apoptosis, ventricular remodeling and cardiac function after AMI in diabetic rats, and explore whether the effect is mediated by hepatocyte growth factor /c-Met signaling pathway. Methods diabetes in 70 male SD rats were induced by intraperitoneal injection with streptozotocin (STZ, 65 mg/kg). After 8 weeks AMI was induced by left anterior descending coronary artery ligation in diabetic rats, and 32 surviving rats were divided into two groups at random: AMI group (n = 16), AMI＋atorvastatin group (n = 16, 20mg·kg-1·d-1). Similar surgical procedure was completed in sham group (n=11) without coronary ligation. atorvastatin was given daily by gavage from the first day after AMI. Two weeks later, the cardiac function, myocardial tissue pathology, myocardial apoptosis, and the expressions of HGF and c-Met were compared among the groups. Results There was no statistical difference on blood glucose, body weight and blood lipids among three groups (P＞0.05). Compared with sham group, AMI group’s LVEF and FS were reduced, LVDd, LVDs, CVF, apoptosis index ， and expressions of HGF and c-Met in mRNA and protein were elevated(P0.05). Compared with sham group, AMI＋atorvastatin group’s LVEF and FS were reduced, LVDd，LVDs，CVF，apoptosis index，and expressions of HGF and c-Met of in mRNA and protein were elevated(P0.05). Compared with AMI group, AMI＋atorvastatin group’s LVEF, FS, and expressions of HGF and c-Met of in mRNA and protein were elevated, LVDd, LVDs, CVF and apoptosis index were reduced(P0.05). Conclusion We can build diabetic rats model complicating AMI successfully, by only intraperitoneal injection of STZ + late ligation of left anteri