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l e t t e r s
Chalk5 encodes a vacuolar H+-translocating
pyrophosphatase influencing grain chalkiness in rice
1 1 1 1 2 1 1 1
Yibo Li , Chuchuan Fan , Yongzhong Xing , Peng Yun , Lijun Luo , Bao Yan , Bo Peng , Weibo Xie ,
1 1 1 1 1
Gongwei Wang , Xianghua Li , Jinghua Xiao , Caiguo Xu Yuqing He
Grain chalkiness is a highly undesirable quality trait in the To fine map the Chalk5 locus, we backcrossed DH27, a double-
. marketing and consumption of rice grain–6. However, haploid line containing the chromosome segment RM593–RM574
d the molecular basis of this trait is poorly understood. from H94 and 55% of the genetic background from Zhenshan 97,
e
v
r Here we show that a major quantitative trait locus (QTL), three times to Zhenshan 97. Self-pollinating the BC F plants hetero-
e 3 1
s Chalk5, influences grain chalkiness, which also affects head zygous for this fragment produced two near-isogenic lines (NILs) in
e
r
s rice yield and many other quality traits. Chalk5 encodes a the Zhenshan 97 background, NIL(ZS97) and NIL(H94). We planted
t +
h vacuolar H -translocating pyrophosphatase (V-PPase) with two BC F populations consisting of 4,373 individuals (population 1)
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