分子生物学张海红10章.ppt

思考题 1.同源重组的特点，功能是什么？ 2.同源重组的两种模型是什么，具体过程如何，二者有什么异同？（看一下动画会更清楚） Holliday模型、DSB模型 3.大肠杆菌的同源重组的蛋白机器（ RecBCD 途径）的作用机制。 4.大肠杆菌和真核生物中在同源重组过程中涉及的关键蛋白有哪些，它们的主要作用是什么？ Change in nuclease activity appears to be inactivation or loss of RecD subunit 3’ overhang could also be coated w/ SSB, interaction of RecBCD and RecA ensures that RecA binds RecBCD pathway Figure 10-5. Steps of DNA processing by RecBCD. Chapter 10 Figure 10-8 Substrates for RecA strand exchange. RecA protein assembles on single-strand DNA and promotes strand invasion Chapter 10 RecA binds to ssDNA and is a strand-exchange protein,