裂蹄木层孔菌发酵产物抑菌活性的研究-中国农学通报.PDF

中国农学通报 2013,29(21):78-82 Chinese Agricultural Science Bulletin 裂蹄木层孔菌发酵产物抑菌活性的研究 邹 莉，谭 昀，杨民宝 （东北林业大学林学院，哈尔滨150040） 摘 要：为了研究裂蹄木层孔菌发酵产物对植物病原菌的抑菌活性。采用菌丝生长速率法测定裂蹄木层 孔菌不同发酵时间的产物对7 种供试病原菌的抑制作用，将裂蹄木层孔菌的发酵产物进行不同的处理， 分级纯化抑菌活性多糖，比较其抑菌活性。结果表明：裂蹄木层孔菌发酵产物对7 种病原菌均有抑制作 用；发酵时间为20 天的裂蹄木层孔菌发酵产物对杨树烂皮病的抑制率最高；裂蹄木层孔菌发酵产物的 主要抑菌活性物质存在于发酵上清液醇提物中；醇沉级别为30%（浓度为50 mg/mL ）醇提物中的多糖对 病原菌的抑制率为供试醇沉级别中最高。说明裂蹄木层孔菌含有抑制病原菌的有效成分，具备成为新 型生物农药的潜力。 关键词：裂蹄木层孔菌；液体发酵；生物农药；抑菌活性 中图分类号：Q939.95 ，TQ458 文献标志码：A 论文编号：2012-2463 Study on the Antimicrobial Activity from Phellinus linteus Fermentation Product Zou Li, Tan Yun, Yang Minbao (CollegeofForestry,NortheastForestryUniversity, Harbin 150040) Abstract: To discover the antifungal activity of the extracts from Phellinus linteus. We used the growth rate method measuring antifungal action to seven kinds of plant pathogen by Phellinus linteus different fermentation time fermentation product, the fermentation product was treated by different way, get the polysaccharide from the ethanol concentration, compare to antifungal activity. The results showed that antibacterial substances from Phellinus linteus had growth inhibitory effects on seven pathogenic bacteria; fermentation time was 20 d, the antibiotic compound had the highest inhibitory rate to Cytospora chry sosperma. The antibiotic compound from Phellinus linteus almost existed in supernatant of acoparia-alcohol extract; 30% alcohol-precipitation concentration (concentration was 50 mg/mL) was highest in all. Phellinus linteus contained the antifungal activity component and had the potential became