D-101型大孔吸附树脂分离纯化细叶鼠曲草总黄酮.PDF

国际中医中药杂志2018年10月第40卷第10期 Int J Trad Chin Med, October 2018, Vol. 40, No.10 ·965 · ·实验与方药 · D-101 型大孔吸附树脂分离纯化 细叶鼠曲草总黄酮 郑梦迪 王勇 汪兴军 张彦 申旭霁 张寒 【摘要】 目的 研究 D-101 型大孔吸附树脂分离纯化细叶鼠曲草总黄酮的最佳工艺条件，并测定 细叶鼠曲草不同部位总黄酮的含量。方法 以细叶鼠曲草总黄酮的吸附率、解吸率为指标，考察 D-101 型大孔吸附树脂对细叶鼠曲草中总黄酮的纯化能力，采用静态吸附法和动态吸附法分析静态饱和吸附量、 静态洗脱率、上样量浓度、上样液 pH 、洗脱液浓度和洗脱液用量对吸附解吸的影响；以芦丁为对照品， 测定总黄酮含量。结果 D-101 型大孔吸附树脂对细叶鼠曲草总黄酮分离纯化效果良好，最佳吸附和解 吸条件为：上样量浓度为 1.0 mg/ml、上样液pH ＝4 、解吸液乙醇浓度为 60%、解吸流速为2.5 BV/h ，在 此条件下细叶鼠曲草总黄酮纯度为 60.92% ；细叶鼠曲草不同部位黄酮含量为：茎叶＞花＞根部。结 论 在最佳纯化分离条件下，D-101 型大孔吸附树脂分离纯化细叶鼠曲草总黄酮纯度提高；细叶鼠曲草 茎叶中黄酮含量最高。 【关键词】 鼠曲草；黄酮；大孔吸附树脂；纯化分离工艺 Study on the techniques of purification flavones from Gnaphalium japonicum Thunb. by D-101 macroporous adsorption resin * Zheng Mengdi , Wang Yong, Wang Xingjun, Zhang Yan, Shen Xuji, * Zhang Han. Department of Pharmacy Xian Medical University, Institute of Medicine, Xian Medical University, Xian 710021, China Corresponding author: Zhang Han, Email: zhanghan11nini@163.com 【Abstract 】 Objective To investigate the optimal isolation and purification conditions of flavonoids in Gnaphlium affine Thunb. and the content of flavonoids in different parts of Gnaphlium affine Thunb. was measured. Methods The separation and purification abilities of D-101 macroporoue adsorbing resins for flavonoids in Gnaphlium affine Thunb. were studied with adorption and desorption as index. The static adsorption and dynamic adsorption methods were used to analyze the effects of static saturated adsorption, static elution rate, sample concentration, sample pH value, eluent concentration and amount of eluent. The flavonoids concentrat