分子生物学（张海红）乳糖操纵子的负调控知识课件.pptVIP

lacZ：编码b －半乳糖苷酶 使乳糖水解 lacY：编码b －半乳糖苷透过酶 使β-半乳糖苷透过细胞壁、质膜进入 细胞内 lacA：编码b －半乳糖苷乙酰转移酶 将乙酰基转移到b －半乳糖苷上 调节基因 lacI：编码阻遏蛋白（repressive protein） 操纵元件Olac （1）28bp的回文结构，可形成十字形结构 （2）这种结构正好与由四个相同亚基组成的阻遏蛋白相匹配。;;The activity of Lac repressor and CAP are controlled allosterically by their signals;Polymerase binding to promoter is enhanced by active CAP;Very low transcription;Second example: araBAD operon ;;;;Third example: galactose operon;;Glu和Gal对Gal操纵子的调节;Fourth example: Tryptophan operon;大肠杆菌色氨酸操纵子的负调控;大肠杆菌色氨酸操纵子的衰减作用：;Four regions (1,2,3,4) of the leader sequence can base pair and form different structures depending on the ribosome action;