产后多虚多瘀动物模型的实验研究-中医妇科学专业论文.docxVIP

成都中医药大学硕士研究生学位论文 成都中医药大学硕士研究生学位论文 PAGE PAGE 1 中文摘要 研究目的：通过对产后大鼠存在的气血亏虚、血瘀状态进行研究，论证此状态 对机体生理、免疫等方面的影响，以论证中医关于“产后多虚多瘀”的理论，指 导产褥期康复保健、预防产后病的发生，为产后病的临床研究和中药新药研究提 供理想的动物模型。 研究方法：将产后大鼠以游泳加放血方式制成“产后多虚多瘀”模型，与未孕 正常组（正常组）、正常产后对照组（产后组）对照，检测产后第 1 天和第 15 天 血常规、血流变的变化；各组大鼠外观表征的变化；肝脏、胸腺脏器系数。 研究结果：1.大鼠产后第一天，红细胞、血红蛋白量、红细胞压积明显低于正 常组（P0.01）；第十五天，模型组 RBC、HGB、HCT 水平继续下降，与正常组及 产后组比较有显著差异（P0.01），产后组已恢复到正常水平，与正常组无显著 差异（P0.05）。2.模型组大鼠肝脏脏器系数明显升高，胸腺系数明显降低，与 正常组比较有显著差异（P0.05）。3.大鼠产后存在气虚、血虚、血瘀的外观表 征，模型组随着实验的进行，症状日渐明显，而产后组症状逐渐消退。 结论：此种方法造模，在一定程度上可以模拟人体“产后多虚多瘀”的病理生 理状态，可为产后病的研究提供良好动物模型。 关键词：产后病 气虚血虚血瘀 动物模型 Abstract Objective: To invest the condition of Qi and blood deficiency of rats after parturition, with this case, to demonstrate the effect of this condition to the organism on physiological functions, immunization and so on. And to authenticate the theory of TCM that “more deficiency and stasis after postpartum”, direct convalescence and health maitenance on puerperium, and provide ideal model for clinical research puerperal disease and researching Chinese medicinal new materials. Methods: Postpartum rats were made of “more deficiency and stasis after postpartum” model through swimming and bloodletting , and compared with Nonfertile group (normal group )and normal parturition control group(puerperal group). To detect blood routine and hemorheological index ，the change of each group on appearance exosyndrome, liver and thymus organ coefficient. Results: 1. On the first day of rats after parturition, the concentration of RBC , HGB and HCT was decreased compared to normal group (P0.01); on the 15th day, the concentration of RBC , HGB and HCT of model group decreased continue, and had significant deviation to the normal group and puerperal group. Puerperal group had reintegrated to normal level. 2. The coefficient of liver organ of model rats heightened obviously, but the thoracic gland coefficient decreased obviously (P0.05). 3. After parturition, rats had appearance of Qi deficiency blood def