猕猴发育过程中肠肝组织生长抑素及其受体表达演变规律-生理学报.PDF

Acta Physiologica Sinica, December 25, 2005, 57 (6): 719-724 719 * 610041 (somatostatin, SST)6 2 d45 d SST SST SST 2 (somatostatin receptor 2, SSTR2) (1)6 SST (27.3 16.6) ng/mg SST SST SST (120.1 35.3) ng/mg 6 (P0.01) (2) SST (3)6 SSTR2 SSTR2 SSTR2 SSTR2 (4)SST SSTR2 SST SST SST SSTR2 SST SST Q483; Q485; R574 Changes of somatostatin and expression of somatostatin receptor in small intestine and liver tissues during macaque development GUO Mei-Mei, TAN Qing-Hua, FAN Hua, HUANG Ming-Hui, WANG Chun-Hui, QIU Xiao-Qing, TANG Cheng-Wei* Division of Peptides Related with Human Diseases, State Key Laboratory of Biotherapy of Human Diseases, West China Hospital, Sichuan University, Chengdu 610041, China Abstract: Intestinal tract, which produces more than fifty kinds of gut peptides, is regarded as the largest endocrine organ. With regard to the gut peptides, a number of studies were focused on their structure, function and the roles in some diseases. The changes in output or distribution of gut peptides in the intestinal tract during development have been largely unknown. This study was aimed to investigate the changes of somatostatin (SST) and somatostatin receptor 2 (SSTR2) in small intestinal and hepatic tissues during the development of macaque. The tissue samples of small intestine, liver or blood samples from peripheral and portal vein of 4 macaques in 6-month fetus, 2-day neonate, 45-day neonate and adult were obtained after anesthetization. The concentrations of SST in blood or tissues of macaques were measured by radioimmunoassay. The distributions of SST in small intestinal or hepatic tissues were visualized by immunohistochemical staining. The expression of SSTR2 was detected by in situ hybridization. SST concentration of intestinal tissue in 6-month-old macaque was (27.3±16.6) ng /mg protein and light positive staining of SST was localized in mucosal crypts but n