益生注射液抗大鼠移植心慢性失功加快模型的机理探讨李明.doc

作者介绍：李明（1973- ），男，主治医师，讲师，博士，主要研究方向：肝胆外科及器官移植。?益生注射液抗大鼠移植心慢性失功加快模型的 作者介绍：李明（1973- ），男，主治医师，讲师，博士， 主要研究方向：肝胆外科及器官移植。 李明1，严律南2 （1、重庆医科大学附属第一医院普外科，重庆 400016； 2、四川大学华西医院普外科，成都 610041） 【摘 要】目的：探讨中药益生注射液抗大鼠移植心慢性失功加快模型的机理，为临床应用提供部分实验依据。方法：采用雌性封闭群SD大鼠20只，分两组，对照组的离体心脏用4℃生理盐水保存3小时后，行心脏移植，移植术后不给任何药物处理，实验组的离体心脏则用含益生注射液的4℃生理盐水保存3小时后，行心脏移植，术后每天腹腔注射益生注射液，剂量为8mg/kg，连续7天。在移植术后第8天，取两组移植心脏，做免疫组化，检测心肌组织中NF-κB、ICAM-1、MHC-II和TGF-β1 分子的表达情况。结果：两组移植心肌组织中均有NF-κB、ICAM-1、MHC-II和TGF-β1 分子的表达，但是实验组和对照组相比较，实验组四种分子的表达明显低于对照组。结论：在缺血再灌注损伤（ischemia reperfusion injury, I/R）诱导的移植心慢性失功加快模型中，益生注射液对移植心有保护作用，其机理可能是减轻I/R损伤所致的炎性级联反应。 【关键词】益生注射液；缺血再灌注损伤；大鼠；移植物慢性失功 【中国图书分类法分类号】R657.3 【文献标识码】A 【收稿日期】2010-05-15 The Possible Molecular Mechanism of Yisheng Injection for Reducing the Progress of A Novel Accelerated Rat Cardiac Graft Failure Model Li Ming, et al （Department of general Surgery, the First Affiliated Hospital, Chongqing Medical University） 【Abstract】Objective: To investigate the possible molecular mechanism of the traditional chinese medicine Yisheng injection, which can reduce the progress of a novel accelerated rat cardiac graft failure model induced by ischemia/reperfusion injury. Methods: 20 female closed colony SD rats were divided into two groups at random. In the control group, the isolated rat hearts were preserved in 0.9% sodium chlorine solution at 4℃ for 3 hours before transplantation, while in the experimental group, the hearts were preserved in 0.9% sodium chlorine solution containing Yisheng injection at 4℃ for 3 hours before transplantation. After transplantation, the experimental group recipients were treated with Yisheng injection 8mg/kg/d for 1 week. Grafts were excised on the 8 day after transplantation to detect the expression of NF-κB, ICAM-1, MHC-II and TGF-β1 , using immunohistochemistry methods. Result: We can detect the expressions of NF-κB, ICAM-1, MHC-II, and TGF-β1 by 8 day after transplantation in two groups. However, the expressions of NF-κB, ICAM-1, MHC-II, and T