牛MyoG基因单核苷酸多态性分析.PDFVIP

安徽农业大学学报, 2013, 40(4): 547-551 Journal of Anhui Agricultural University 网络出版时间：2013-7-12 10:26:01 [URL] /kcms/detail/34.1162.S1026.009.html 牛MyoG 基因单核苷酸多态性分析 1 1, 2 1 1 1, 2 1, 2 * 杨漫漫 ，刘洪瑜 ，王 恒 ，王力生 ，章孝荣 ，刘旭光 (1. 安徽农业大学动物科技学院，合肥 230036 ；2. 安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036) 摘 要：以皖东牛和广丰牛共128 头牛为研究对象，采用PCR-RFLP 方法检测牛MyoG 基因的多态性。结果表 明，在牛MyoG 基因的外显子466 碱基处发生GC 突变，检测到AA 和AB 2 种基因型，该位点的突变未引起氨 基酸序列的变化。在皖东牛群体中AA 基因型频率为23.66% ，AB 基因型频率为76.34%；在广丰牛群体中AA 基 因型频率为17.24%，AB 基因型频率为82.76%。皖东牛的多态信息含量（PIC ）为0.3606 ，广丰牛为0.3702 ，在该 位点均处于中度多态。卡方检验表明皖东牛群体处于哈代-温伯格不平衡状态(P0. 05)；广丰牛处于哈代-温伯格平 衡状态(P0. 05) 。 关键词：牛；MyoG 基因；多态性 中图分类号：S823.2 文献标识码：A 文章编号：1672−352X (2013)04−0547−05 Single nucleotide polymorphism analysis of MyoG gene in cattle 1 1,2 1 1 1,2 1, 2 YANG Man-man , LIU Hong-yu , WANG Heng , WANG Li-sheng , ZHANG Xiao-rong , LIU Xu-guang (1. School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036; 2. Anhui Provincial Key Laboratory of Local Livestock and Poultry Genetic Resources Conservation and BioBreeding, Hefei 230036) Abstract: One hundred and twenty-eight cattles from Wandong and Guangfeng populations were used to de- tect the polymorphism of MyoG gene by PCR-RFLP method. The results show that a GC mutation was occur in 466 base position of exon 1, and 2 types of genotypes (AA and AB) were found. The mutation had not caused the change of amino acid. Genotypic frequencies of AA and AB are 23.66% and 76.34% respectively in Wandong population, the genotypic frequency of AA is 17.24% and AB is 82.76% in Guangfeng population. The polymor- phism information content (PIC) is 0.3606 in Wandong po