《氨氧化细菌的培养与特性研究论文》-毕业学术论文设计.docVIP

l l l 毕 业 论 文 题目： 氨氧化细菌的培养与特性研究 学 生： 学 号： 200803040117 院 （系）： 资源与环境学院 专 业： 环 境 工 程 指导教师： 2012 年06月13日 氨氧化细菌的培养与特性研究 摘 要 本研究中采用SBR反应器，通过控制进水成分及反应器的运行条件，如曝气时间，反应周期等，在亚硝态氮有一定的积累情况下，并提取分离细菌，研究在氨氧化细菌富集颗粒污泥的作用下，不同PH对氨氧化过程的影响，为氨氧化过程的特征分析提供了依据。最后通过分离，筛选出优势氨氧化细菌，并通过观察它菌落的形状，颜色，以及革兰氏染色并分类鉴定它的种属。其中驯化培养成熟的颗粒污泥呈黄褐色，颜色较浅，粒径在0.4～3.5mm之间。对温度较敏感，气温较低时(冬季)细菌的活性明显降低，其最适温度为20～30℃， 夏季时生物的活性较高。用于培养颗粒污泥的SBR反应器能够稳定运行，氨氮的转化率可达到80％以上，并有效地实现NO-2-N累积，驯化后的活性污泥在相应pH=8条件下具有氨氧化能力最强，氨氧化细菌的活性强,并分离出具有硝化能力的氨氧化细菌。 关键字： 序批式反应器，氨氧化细菌，富集污泥，短程硝化 Study on the Ammonium-oxidizing bacteria of culture ABSTRACT The article through controlling substrate concentration and the operation of the reactor conditions training ammonium oxidation bacteria enrichment particles in SBR reactor; In the nitrate accumulation of a certain, and extraction and separation, research in ammonium oxidation bacteria enrichment granular sludge under the action of different PH of ammonia oxidation process, the effects of the ammonium oxidation process for the characteristic analysis to provide the basis. Finally through the separation, and screened advantage ammonium oxidation bacteria, and by watching its colonies shape, color, and the classification of its species. The major research conclusions are as follows: Mature granular sludge was brown，lighter in color,and its diameter was between O.4-3.5mm．It was sensitive to temperature．The activity of bacteria was significantly decreased at low temperature(in winter)as the optimum temperature of 20～30℃．while the biological activity was higher in summer． SBR reactor used to cultivate granular sludge operated stably．Ammonia degradationrate reached 80％，and N02-N has been accumulated effectively . The acclimated active sludge had capacity of oxidize ammonia under the pH 8 factor，Ammonium oxidation of bacteria strong activity. And isolated nitrification ability of ammonia oxidation bacteria KEYWORDS：Sequencing Batch Reactor，Ammonium—oxid