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摘要
Abstract
Asan materialfor acid,has
importantsynthetic drugcompounds,(-)shikimic
been usedin andother
widely chemical,food,medicinefields.Therefore,Thestudy
andits and has
acidresourcesextraction,separationpurification
on(-)shikimic
this and
culture,fermentationverification,three
importantsignificance.Inpaper,after
ten
whichCall shikimicacidwerescreenedfrommorethan soil
strains produce
0.1
acidofstrainsfromthreesoil was
samples,theyieldof(-)shikimic samples 74班,
be and of
0,131 canusedforthe
g/L,0.087g/L.Sothey developmentapplication
fermentation.Thedesked in formwereobtained
pure by
subsequent products
NMRandElementalwereusedto
recrystallizationmethod,IR,MS,1H analysis
characterizethe
products.
strainsthat
Inthis conditionsofthe
paper,fermentation
andwerescreenedfromsoil were bestfermentation
acid samplesoptimized.the
werethat:theinitialvalueoffermentationwas
conditions pH liquid7.0,inoculums
was volumewas100 timewas91 h,under
5%,fermentation mL,fermentation
liquid
ofshikimicisO.946
the content
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